ArticleThe Journal of biological chemistry2011
In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Cx45 regulation by kinases and impact of expression in heart failure.Journal of molecular and cellular cardiology · 2025Article
- Overexpression of Cx43: Is It an Effective Approach for the Treatment of Cardiovascular Diseases?Biomolecules · 2025Review
- Spatial and Temporal Localization of Connexins in Cells Using Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Cooperative cell-cell actin network remodeling to perform Gap junction endocytosis.Basic and clinical andrology · 2023Article
- Regulation of Cx43 Gap Junction Intercellular Communication by Bruton's Tyrosine Kinase and Interleukin-2-Inducible T-Cell Kinase.Biomolecules · 2023Article
- The roles of connexins and gap junctions in the progression of cancer.Cell communication and signaling : CCS · 2023Review
- Expression of connexins and pannexins in diseased human liver.EXCLI journal · 2022Article
- cAMP controls a trafficking mechanism that maintains the neuron specificity and subcellular placement of electrical synapses.Developmental cell · 2021Article
- Connexins in the Heart: Regulation, Function and Involvement in Cardiac Disease.International journal of molecular sciences · 2021Review
- Connexin 43 maintains tissue polarity and regulates mitotic spindle orientation in the breast epithelium.Journal of cell science · 2019Article
- Connexin43 Carboxyl-Terminal Domain Directly Interacts with β-Catenin.International journal of molecular sciences · 2018Article
- Intracellular trafficking pathways of Cx43 gap junction channels.Biochimica et biophysica acta. Biomembranes · 2018Review
- GJA1-20k Arranges Actin to Guide Cx43 Delivery to Cardiac Intercalated Discs.Circulation research · 2017Article
- Mechanism of action of the anti-inflammatory connexin43 mimetic peptide JM2.American journal of physiology. Cell physiology · 2017Article
- Article
- Connexin Type and Fluorescent Protein Fusion Tag Determine Structural Stability of Gap Junction Plaques.The Journal of biological chemistry · 2015Article
- CANDLES, an assay for monitoring GPCR induced cAMP generation in cell cultures.Cell communication and signaling : CCS · 2014Article
- Glucose regulates mitochondrial motility via Milton modification by O-GlcNAc transferase.Cell · 2014Article
- Trafficking highways to the intercalated disc: new insights unlocking the specificity of connexin 43 localization.Cell communication & adhesion · 2014Review
- Reconstituting the motility of isolated intracellular cargoes.Methods in enzymology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Trafficking of the proteins that form gap junctions (connexins) from the site of synthesis to the junctional domain appears to require cytoskeletal delivery mechanisms. Although many cell types exhibit specific delivery of connexins to polarized cell sites, such as connexin32 (Cx32) gap junctions specifically localized to basolateral membrane domains of hepatocytes, the precise roles of actin- and tubulin-based systems remain unclear. We have observed fluorescently tagged Cx32 trafficking linearly at speeds averaging 0.25 μm/s in a polarized hepatocyte cell line (WIF-B9), which is abolished by 50 μM of the microtubule-disrupting agent nocodazole. To explore the involvement of cytoskeletal components in the delivery of connexins, we have used a preparation of isolated Cx32-containing vesicles from rat hepatocytes and assayed their ATP-driven motility along stabilized rhodamine-labeled microtubules in vitro. These assays revealed the presence of Cx32 and kinesin motor proteins in the same vesicles. The addition of 50 μM ATP stimulated vesicle motility along linear microtubule tracks with velocities of 0.4-0.5 μm/s, which was inhibited with 1 mM of the kinesin inhibitor AMP-PNP (adenylyl-imidodiphosphate) and by anti-kinesin antibody but only minimally affected by 5 μM vanadate, a dynein inhibitor, or by anti-dynein antibody. These studies provide evidence that Cx32 can be transported intracellularly along microtubules and presumably to junctional domains in cells and highlight an important role of kinesin motor proteins in microtubule-dependent motility of Cx32.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.