ArticleMolecular and cellular biology2011
Mechanistic analysis of a DNA damage-induced, PTEN-dependent size checkpoint in human cells.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Silencing PTEN in the fallopian tube promotes enrichment of cancer stem cell-like function through loss of PAX2.Cell death & disease · 2021Article
- PTEN: A Thrifty Gene That Causes Disease in Times of Plenty?Frontiers in nutrition · 2020Article
- Genetic variations in the PI3K/PTEN/AKT/mTOR pathway predict tumor response and disease-free survival in locally advanced rectal cancer patients receiving preoperative chemoradiotherapy and radical surgery.Journal of Cancer · 2018Article
- Cell Cycle Control by PTEN.Journal of molecular biology · 2017Review
- Epithelial protein lost in neoplasm (EPLIN): Beyond a tumor suppressor.Genes & diseases · 2017Review
- Correction of PTEN mutations in glioblastoma cell lines via AAV-mediated gene editing.PloS one · 2017Article
- Medical treatment for gastro-entero-pancreatic neuroendocrine tumours.World journal of gastrointestinal oncology · 2016Review
- Phosphatase and Tensin Homolog Is a Growth Repressor of Both Rhizoid and Gametophore Development in the Moss Physcomitrella patens.Plant physiology · 2015Article
- EPLIN: a fundamental actin regulator in cancer metastasis?Cancer metastasis reviews · 2015Review
- A new class of cancer-associated PTEN mutations defined by membrane translocation defects.Oncogene · 2015Article
- miR-494-3p Regulates Cellular Proliferation, Invasion, Migration, and Apoptosis by PTEN/AKT Signaling in Human Glioblastoma Cells.Cellular and molecular neurobiology · 2015Article
- A chemogenomic screening identifies CK2 as a target for pro-senescence therapy in PTEN-deficient tumours.Nature communications · 2015Article
- PTEN is a negative regulator of NK cell cytolytic function.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Mutational and functional analysis of the tumor-suppressor PTPRD in human melanoma.Human mutation · 2014Article
- A mitochondrial origin for frontotemporal dementia and amyotrophic lateral sclerosis through CHCHD10 involvement.Brain : a journal of neurology · 2014Article
- Subcellular targeting and dynamic regulation of PTEN: implications for neuronal cells and neurological disorders.Frontiers in molecular neuroscience · 2014Review
- PI3K-AKT-mTOR-signaling and beyond: the complex network in gastroenteropancreatic neuroendocrine neoplasms.Theranostics · 2014Review
- Counteracting the activation of pAkt by inhibition of MEK/Erk inhibition reduces actin disruption-mediated apoptosis in PTEN-null PC3M prostate cancer cell lines.Oncology letters · 2013Article
- Overexpression of phosphatase and tensin homolog improves fitness and decreases Plasmodium falciparum development in Anopheles stephensi.Microbes and infection · 2013Article
- PTEN at a glance.Journal of cell science · 2012Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
Following DNA damage, human cells undergo arrests in the G(1) and G(2) phases of the cell cycle and a simultaneous arrest in cell size. We previously demonstrated that the cell size arrest can be uncoupled from the cell cycle arrest by mutational inactivation of the PTEN tumor suppressor gene. Here we show that the cell size checkpoint is inducible by DNA-damaging chemotherapeutic agents as well as by ionizing radiation and is effectively regulated by PTEN but not by its oncogenic counterpart, PIK3CA. Mutational analysis of PTEN and pharmacological inhibition of Akt revealed that modulation of Akt phosphorylation is unnecessary for cell size checkpoint control. To discover putative PTEN regulators and/or effectors involved in size checkpoint control, we employed a novel endogenous epitope tagging (EET) approach, which revealed that endogenous PTEN interacts at the membrane with an actin-remodeling complex that includes actin, gelsolin, and EPLIN. Pharmacological inhibition of actin remodeling in PTEN(+/+) cells recapitulated the lack of size checkpoint control seen in PTEN(-/-) cells. Taken together, these results provide further support for the existence of a DNA damage-inducible size checkpoint that is regulated by a major tumor suppressor, and they provide a novel Akt-independent mechanism by which PTEN controls cell size.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.