ArticleIEEE transactions on bio-medical engineering2011
A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with di-4-ANEPPS using pulsed LED excitation.
Article in IEEE transactions on bio-medical engineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- A Beginner's Guide to Cardiac Optical Mapping.Cardiovascular engineering and technology · 2026Review
- Combined emission ratiometry and motion tracking for optical mapping of contracting hearts: Validation with monophasic action potentials.The Journal of physiology · 2026Article
- Optical Mapping of Pacing-Elicited Slow Waves in the Swine Stomach: Role of Virtual Electrodes.Neurogastroenterology and motility · 2026Article
- Panoramic voltage-sensitive optical mapping of contracting hearts using cooperative multiview motion tracking with 12 cameras.The Journal of physiology · 2026Article
- Optical mapping of cardiac electromechanics in beating in vivo hearts.Biophysical journal · 2023Article
- Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy.Chemical & biomedical imaging · 2023Article
- Optical mapping of contracting hearts.The Journal of physiology · 2023Review
- Living myocardial slices: Advancing arrhythmia research.Frontiers in physiology · 2023Review
- Ectopic foci do not co-locate with ventricular epicardial stretch during early acute regional ischemia in isolated pig hearts.Physiological reports · 2022Article
- Basic Research Approaches to Evaluate Cardiac Arrhythmia in Heart Failure and Beyond.Frontiers in physiology · 2022Review
- Real-Time Optical Mapping of Contracting Cardiac Tissues With GPU-Accelerated Numerical Motion Tracking.Frontiers in cardiovascular medicine · 2022Article
- Methodology for Cross-Talk Elimination in Simultaneous Voltage and Calcium Optical Mapping Measurements With Semasbestic Wavelengths.Frontiers in physiology · 2022Article
- Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review.Frontiers in physiology · 2021Review
- Cardiac Optical Mapping in Situ in Swine Models: A View of the Current Situation.Medicina (Kaunas, Lithuania) · 2020Review
- Cardiac optical mapping - State-of-the-art and future challenges.The international journal of biochemistry & cell biology · 2020Review
- Recent progress in optical voltage-sensor technology and applications to cardiac research: from single cells to whole hearts.Progress in biophysics and molecular biology · 2020Review
- Optical mapping of the pig heart in situ under artificial blood circulation.Scientific reports · 2020Article
- Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel, Switzerland) · 2020Review
- Optical mapping of electromechanics in intact organs.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- High-Resolution Optical Measurement of Cardiac Restitution, Contraction, and Fibrillation Dynamics in Beating vs. Blebbistatin-Uncoupled Isolated Rabbit Hearts.Frontiers in physiology · 2020Article
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6 authors.
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Abstract
We developed a new method for ratiometric optical mapping of transmembrane potential (V(m)) in cardiac preparations stained with di-4-ANEPPS. V(m)-dependent shifts of excitation and emission spectra establish two excitation bands (<481 and >481 nm) that produce fluorescence changes of opposite polarity within a single emission band (575-620 nm). The ratio of these positive and negative fluorescence signals (excitation ratiometry) increases V(m) sensitivity and removes artifacts common to both signals. We pulsed blue (450 ± 10 nm) and cyan (505 ± 15 nm) light emitting diodes (LEDs) at 375 Hz in alternating phase synchronized to a camera (750 frames-per-second). Fluorescence was bandpass filtered (585 ± 20 nm). This produced signals with upright (blue) and inverted (cyan) action potentials (APs) interleaved in sequential frames. In four whole swine hearts with motion chemically arrested, fractional fluorescence for blue, cyan, and ratio signals was 1.2 ± 0.3%, 1.2 ± 0.3%, and 2.4 ± 0.6%, respectively. Signal-to-noise ratios were 4.3 ± 1.4, 4.0 ± 1.2, and 5.8 ± 1.9, respectively. After washing out the electromechanical uncoupling agent, we characterized motion artifact by cross-correlating blue, cyan, and ratio signals with a signal with normal AP morphology. Ratiometry improved cross-correlation coefficients from 0.50 ± 0.48 to 0.81 ± 0.25, but did not cancel all motion artifacts. These findings demonstrate the feasibility of pulsed LED excitation ratiometry in myocardium.
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