ArticleExperimental cell research2011
Migration of connective tissue-derived cells is mediated by ultra-low concentration gradient fields of EGF.
Article in Experimental cell research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- The Charming World of the Extracellular Matrix: A Dynamic and Protective Network of the Intestinal Wall.Frontiers in medicine · 2021Review
- Microfluidic and Microscale Assays to Examine Regenerative Strategies in the Neuro Retina.Micromachines · 2020Review
- A Micro-Optic Stalk (μOS) System to Model the Collective Migration of Retinal Neuroblasts.Micromachines · 2020Article
- A localized surface acoustic wave applied spatiotemporally controllable chemical gradient generator.Biomicrofluidics · 2020Article
- A novel electro-chemotactic approach to impact the directional migration of transplantable retinal progenitor cells.Experimental eye research · 2019Article
- A Milled Microdevice to Advance Glia-Mediated Therapies in the Adult Nervous System.Micromachines · 2019Article
- Collective behaviors of Drosophila-derived retinal progenitors in controlled microenvironments.PloS one · 2019Article
- Controlled microenvironments to evaluate chemotactic properties of cultured Müller glia.Experimental eye research · 2018Article
- Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor.Lasers in medical science · 2018Article
- Cellular and Molecular Mediators of Intestinal Fibrosis.Journal of Crohn's & colitis · 2017Review
- In vitro formation of neuroclusters in microfluidic devices and cell migration as a function of stromal-derived growth factor 1 gradients.Cell adhesion & migration · 2017Article
- Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals.Lasers in medical science · 2017Article
- Controlled microfluidics to examine growth-factor induced migration of neural progenitors in the Drosophila visual system.Journal of neuroscience methods · 2016Article
- Predicted molecular signaling guiding photoreceptor cell migration following transplantation into damaged retina.Scientific reports · 2016Article
- Article
- EGF as a New Therapeutic Target for Medulloblastoma Metastasis.Cellular and molecular bioengineering · 2015Article
- Diffusion phenomena of cells and biomolecules in microfluidic devices.Biomicrofluidics · 2015Article
- Effect of epidermal growth factor (EGF) on the phosphorylation of mitogen-activated protein kinase (MAPK) in the bovine oviduct in vitro: Alteration by heat stress.The Journal of reproduction and development · 2015Article
- Article
- Role of Epidermal Growth Factor-Triggered PI3K/Akt Signaling in the Migration of Medulloblastoma-Derived Cells.Cellular and molecular bioengineering · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The directed migration of cells towards chemical stimuli incorporates simultaneous changes in both the concentration of a chemotactic agent and its concentration gradient, each of which may influence cell migratory response. In this study, we utilized a microfluidic system to examine the interactions between epidermal growth factor (EGF) concentration and EGF gradient in stimulating the chemotaxis of connective tissue-derived fibroblast cells. Cells seeded within microfluidic devices were exposed to concentration gradients established by EGF concentrations that matched or exceeded those required for maximum chemotactic responses seen in transfilter migration assays. The migration of individual cells within the device was measured optically after steady-state gradients had been experimentally established. Results illustrate that motility was maximal at EGF concentration gradients between .01- and 0.1-ng/(mL.mm) for all concentrations used. In contrast, the number of motile cells continually increased with increasing gradient steepness for all concentrations examined. Microfluidics-based experiments exposed cells to minute changes in EGF concentration and gradient that were in line with the acute EGFR phosphorylation measured. Correlation of experimental data with established mathematical models illustrated that the fibroblasts studied exhibit an unreported chemosensitivity to minute changes in EGF concentration, similar to that reported for highly motile cells, such as macrophages. Our results demonstrate that shallow chemotactic gradients, while previously unexplored, are necessary to induce the rate of directed cellular migration and the number of motile cells in the connective tissue-derived cells examined.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.