ArticleCurrent microbiology2011
16S rRNA gene sequence analysis of drinking water using RNA and DNA extracts as targets for clone library development.
Article in Current microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- Dynamics of the Microbial Community and Opportunistic Pathogens after Water Stagnation in the Premise Plumbing of a Building.Microbes and environments · 2022Article
- The Bacterial Community Diversity of Bathroom Hot Tap Water Was Significantly Lower Than That of Cold Tap and Shower Water.Frontiers in microbiology · 2021Article
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- The Role of Pseudomonas in Heterotrophic Nitrification: A Case Study on Shrimp Ponds (Microorganisms · 2019Article
- Multiscale investigation of a symbiotic microalgal-integrated fixed film activated sludge (MAIFAS) process for nutrient removal and photo-oxygenation.Bioresource technology · 2018Article
- Bacterial abundance and diversity in pond water supplied with different feeds.Scientific reports · 2016Article
- Biofilms on Hospital Shower Hoses: Characterization and Implications for Nosocomial Infections.Applied and environmental microbiology · 2016Article
- Vertical Distribution of Functional Potential and Active Microbial Communities in Meromictic Lake Kivu.Microbial ecology · 2015Article
- Distribution of human-specific bacteroidales and fecal indicator bacteria in an urban watershed impacted by sewage pollution, determined using RNA- and DNA-based quantitative PCR assays.Applied and environmental microbiology · 2015Article
- Bacterial DNA sifted from the Trichoplax adhaerens (Animalia: Placozoa) genome project reveals a putative rickettsial endosymbiont.Genome biology and evolution · 2013Article
- Microbial community dynamics of an urban drinking water distribution system subjected to phases of chloramination and chlorination treatments.Applied and environmental microbiology · 2012Article
- Metagenomic analyses of drinking water receiving different disinfection treatments.Applied and environmental microbiology · 2012Article
- A biofilm model developed to investigate survival and disinfection of Mycobacterium mucogenicum in potable water.Biofouling · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial composition of chlorinated drinking water was analyzed using 16S rRNA gene clone libraries derived from DNA extracts of 12 samples and compared to clone libraries previously generated using RNA extracts from the same samples. Phylogenetic analysis of 761 DNA-based clone sequences showed that unclassified bacteria were the most abundant group, representing nearly 62% of all DNA sequences analyzed. Other phylogenetic groups identified included Proteobacteria (20%), Actinobacteria (9%), Cyanobacteria (4%), and Bacteroidetes (2%). The composition of RNA-based libraries (1122 sequences) was similar to the DNA-based libraries with a few notable exceptions: Proteobacteria were more dominant in the RNA clone libraries (i.e., 35% RNA; 20% DNA). Differences in the Proteobacteria composition were also observed; alpha-Proteobacteria was 22 times more abundant in the RNA-based clones while beta-Proteobacteria was eight times more abundant in the DNA libraries. Nearly twice as many DNA operational taxonomic units (OTUs) than RNA OTUs were observed at distance 0.03 (101 DNA; 53 RNA). Twenty-four OTUs were shared between all RNA- and DNA-based libraries (OTU0.03) representing only 18% of the total OTUs, but 81% (1527/1883) of all sequences. Such differences between clone libraries demonstrate the necessity of generating both RNA- and DNA-derived clone libraries to compare these two different molecular approaches for community analyses.
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