ArticlePloS one2011
RNA captor: a tool for RNA characterization.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs.Nucleic acids research · 2017Article
- Dumbbell-PCR: a method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences.Nucleic acids research · 2015Article
- Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature tRNA.RNA biology · 2015Article
- Capping of vesicular stomatitis virus pre-mRNA is required for accurate selection of transcription stop-start sites and virus propagation.Nucleic acids research · 2014Article
- High-throughput sequencing and de novo assembly of Brassica oleracea var. Capitata L. for transcriptome analysis.PloS one · 2014Article
- Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase - engineering a thermostable ATP independent enzyme.BMC molecular biology · 2012Article
- Analysis of expressed sequence tags generated from full-length enriched cDNA libraries of melon.BMC genomics · 2011Article
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1 author.
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Abstract
backgroundIn the genome era, characterizing the structure and the function of RNA molecules remains a major challenge. Alternative transcripts and non-protein-coding genes are poorly recognized by the current genome-annotation algorithms and efficient tools are needed to isolate the less-abundant or stable RNAs.
resultsA universal RNA-tagging method using the T4 RNA ligase 2 and special adapters is reported. Based on this system, protocols for RACE PCR and full-length cDNA library construction have been developed. The RNA tagging conditions were thoroughly optimized and compared to previous methods by using a biochemical oligonucleotide tagging assay and RACE PCRs on a range of transcripts. In addition, two large-scale full-length cDNA inventories relying on this method are presented.
conclusionThe RNA Captor is a straightforward and accessible protocol. The sensitivity of this approach was shown to be higher compared to previous methods, and applicable on messenger RNAs, non-protein-coding RNAs, transcription-start sites and microRNA-directed cleavage sites of transcripts. This strategy could also be used to study other classes of RNA and in deep sequencing experiments.
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