ArticlePloS one2011
A PiggyBac-based recessive screening method to identify pluripotency regulators.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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Who cites it
39 citing papers in PubMed.
- Integration of the naive pluripotency gene network with the response to GSK3 inhibition by Tcf7l1-driven enhancer decommissioning.Stem cell reports · 2026Article
- SRRM2 splicing factor modulates cell fate in early development.Biology open · 2024Article
- Cooperative genetic networks drive embryonic stem cell transition from naïve to formative pluripotency.The EMBO journal · 2021Article
- Zfp281 orchestrates interconversion of pluripotent states by engaging Ehmt1 and Zic2.The EMBO journal · 2020Article
- Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency.Cell stem cell · 2019Article
- Capacitation of human naïve pluripotent stem cells for multi-lineage differentiation.Development (Cambridge, England) · 2019Article
- Tracing the transitions from pluripotency to germ cell fate with CRISPR screening.Nature communications · 2018Article
- Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 Regulation in Naive Pluripotency Maintenance and Dissolution.Cell reports · 2018Article
- TCF7L1 suppresses primitive streak gene expression to support human embryonic stem cell pluripotency.Development (Cambridge, England) · 2018Article
- Article
- The Art of Capturing Pluripotency: Creating the Right Culture.Stem cell reports · 2017Review
- Noncanonical function of DGCR8 controls mESC exit from pluripotency.The Journal of cell biology · 2017Article
- Convergence of cMyc and β-catenin on Tcf7l1 enables endoderm specification.The EMBO journal · 2016Article
- Signalling Through Retinoic Acid Receptors is Required for Reprogramming of Both Mouse Embryonic Fibroblast Cells and Epiblast Stem Cells to Induced Pluripotent Stem Cells.Stem cells (Dayton, Ohio) · 2015Article
- LIF signal in mouse embryonic stem cells.JAK-STAT · 2015Review
- Mapping the route from naive pluripotency to lineage specification.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Genetic exploration of the exit from self-renewal using haploid embryonic stem cells.Cell stem cell · 2014Article
- A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.PloS one · 2014Article
- Gene regulatory networks mediating canonical Wnt signal-directed control of pluripotency and differentiation in embryo stem cells.Stem cells (Dayton, Ohio) · 2013Article
- A competitive protein interaction network buffers Oct4-mediated differentiation to promote pluripotency in embryonic stem cells.Molecular systems biology · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Phenotype driven genetic screens allow unbiased exploration of the genome to discover new biological regulators. Bloom syndrome gene (Blm) deficient embryonic stem (ES) cells provide an opportunity for recessive screening due to frequent loss of heterozygosity. We describe a strategy for isolating regulators of mammalian pluripotency based on conversion to homozygosity of PiggyBac gene trap insertions combined with stringent selection for differentiation resistance. From a screen of 2000 mutants we obtained a disruptive integration in the Tcf3 gene. Homozygous Tcf3 mutants showed impaired differentiation and enhanced self-renewal. This phenotype was reverted in a dosage sensitive manner by excision of one or both copies of the gene trap. These results provide new evidence confirming that Tcf3 is a potent negative regulator of pluripotency and validate a forward screening methodology to identify modulators of pluripotent stem cell biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.