ArticleMolecular & cellular proteomics : MCP2011
Nanodisc-based co-immunoprecipitation for mass spectrometric identification of membrane-interacting proteins.
Article in Molecular & cellular proteomics : MCP, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Recent advances in membrane mimetics for membrane protein research.Biochemical Society transactions · 2023Review
- Improved Immunoprecipitation to Mass Spectrometry Method for the Enrichment of Low-Abundant Protein Targets.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Nanodiscs and Mass Spectrometry: Making Membranes Fly.International journal of mass spectrometry · 2020Article
- Regulation of Membrane Calcium Transport Proteins by the Surrounding Lipid Environment.Biomolecules · 2019Review
- Nanodiscs in Membrane Biochemistry and Biophysics.Chemical reviews · 2017Review
- Probing the Lipid Annular Belt by Gas-Phase Dissociation of Membrane Proteins in Nanodiscs.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2016Article
- Probing the Lipid Annular Belt by Gas-Phase Dissociation of Membrane Proteins in Nanodiscs.Angewandte Chemie (International ed. in English) · 2016Article
- Nanodisc-solubilized membrane protein library reflects the membrane proteome.Analytical and bioanalytical chemistry · 2013Article
- Nanodiscs as a new tool to examine lipid-protein interactions.Methods in molecular biology (Clifton, N.J.) · 2013Review
- Ice breaking in GPCR structural biology.Acta pharmacologica Sinica · 2012Review
- Ultra-thin layer MALDI mass spectrometry of membrane proteins in nanodiscs.Analytical and bioanalytical chemistry · 2012Article
- Proteomic identification and validation of novel interactions of the putative tumor suppressor PRELP with membrane proteins including IGFI-R and p75NTR.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteomic identification of protein interactions with membrane associated molecules in their native membrane environment pose a challenge because of technical problems of membrane handling. We investigate the possibility of employing membrane nanodiscs for harboring the membrane associated molecule to tackle the challenges. Nanodiscs are stable, homogenous pieces of membrane with a discoidal shape. They are stabilized by an encircling amphipatic protein with an engineered epitope tag. In the present study we employ the epitope tag of the nanodiscs for detection and co-immunoprecipitation of interaction partners of the glycolipid ganglioside GM1 harbored by nanodiscs. Highly specific binding activity for nanodisc-GM1 immobilized on sensorchips was observed by surface plasmon resonance in culture media from enterotoxigenic Escherischia coli. To isolate the interaction partner(s) from enterotoxigenic Escherischia coli, GM1-nanodiscs were employed for co-immunoprecipitation. The B subunit of heat labile enterotoxin was identified as a specific interaction partner by mass spectrometry, thus demonstrating that nanodisc technology is useful for highly specific detection and identification of interaction partners to specific lipids embedded in a membrane bilayer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.