ArticleApplied and environmental microbiology2011
Increased fitness and alteration of metabolic pathways during Bacillus subtilis evolution in the laboratory.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- Simultaneous optimization of lignocellulosic sugar catabolism via systematic laboratory evolution under complex selection pressure.Nature communications · 2026Article
- Toward a Functional Genetics of Adaptation: Insights From Microbial Experimental Evolution.Genome biology and evolution · 2026Review
- Characterising the Multiple-Plastic Degrading Strain of Bacillus subtilis GM_03 From the Galleria mellonella Microbiome.Environmental microbiology reports · 2025Article
- Lacticaseibacillus paracasei AD22 Stress Response in Brined White Cheese Matrix: In Vitro Probiotic Profiles and Molecular Characterization.Probiotics and antimicrobial proteins · 2025Article
- Evolving a plant-beneficial bacterium in soil vs. nutrient-rich liquid culture has contrasting effects on in-soil fitness.Applied and environmental microbiology · 2025Article
- Molecular basis of the phenotypic variants arising from aMicrobial genomics · 2023Article
- Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China.BMC microbiology · 2022Article
- Environmental Dependence of Competitive Fitness in Rifampin-ResistantApplied and environmental microbiology · 2022Article
- Impact of Rap-Phr system abundance on adaptation of Bacillus subtilis.Communications biology · 2021Article
- Rampant loss of social traits during domestication of a Bacillus subtilis natural isolate.Scientific reports · 2020Article
- From Root to Tips: Sporulation Evolution and Specialization in Bacillus subtilis and the Intestinal Pathogen Clostridioides difficile.Molecular biology and evolution · 2019Article
- Article
- Can the experimental evolution programme help us elucidate the genetic basis of adaptation in nature?Molecular ecology · 2016Review
- Article
- Experimental evolution of enhanced growth by Bacillus subtilis at low atmospheric pressure: genomic changes revealed by whole-genome sequencing.Applied and environmental microbiology · 2015Article
- Thermal and solvent stress cross-tolerance conferred to Corynebacterium glutamicum by adaptive laboratory evolution.Applied and environmental microbiology · 2015Article
- Sporulation during growth in a gut isolate of Bacillus subtilis.Journal of bacteriology · 2014Article
- Exposure of Bacillus subtilis to low pressure (5 kilopascals) induces several global regulons, including those involved in the SigB-mediated general stress response.Applied and environmental microbiology · 2014Article
- Monitoring intraspecies competition in a bacterial cell population by cocultivation of fluorescently labelled strains.Journal of visualized experiments : JoVE · 2014Article
- Nitrogen gas flushing can be bactericidal: the temperature-dependent destiny of Bacillus weihenstephanensis KBAB4 under a pure N2 atmosphere.Frontiers in microbiology · 2014Article
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Five batch cultures of Bacillus subtilis were subjected to evolution in the laboratory for 6,000 generations under conditions repressing sporulation in complex liquid medium containing glucose. Between generations 1,000 and 2,000, variants with a distinct small-colony morphology arose and swept through four of the five populations that had been previously noted for their loss of sporulation (H. Maughan et al., Genetics 177:937-948, 2007). To better understand the nature of adaptation in these variants, individual strains were isolated from one population before (WN715) and after (WN716) the sweep. In addition to colony morphology, strains WN715 and WN716 differed in their motility, aerotaxis, and cell morphology. Competition experiments showed that strain WN716 had evolved a distinct fitness advantage over the ancestral strain and strain WN715 during growth and the transition to the postexponential growth phase, which was more pronounced when WN715 was present in the coculture. Microarray analyses revealed candidate genes in which mutations may have produced some of the observed phenotypes. For example, loss of motility in WN716 was accompanied by decreased transcription of all flagellar, motility, and chemotaxis genes on the microarray. Transcription of alsS and alsD was also lower in strain WN716, and the predicted loss of acetoin production and enhanced acetate production was confirmed by high-performance liquid chromatography (HPLC) analysis. The results suggested that the derived colony morphology of strain WN716 was associated with increased fitness, the alteration of several metabolic pathways, and the loss of a typical postexponential-phase response.
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