Evidence map›Paper›PMID 21531829›Full record

ArticleApplied and environmental microbiology2011

Molecular characterization of Cronobacter lipopolysaccharide O-antigen gene clusters and development of serotype-specific PCR assays.

K G Jarvis, C J Grim, A A Franco, G Gopinath, V Sathyamoorthy, L Hu, J A Sadowski, C S Lee, B D Tall

Abstract readEvaluation Study
In one paragraph

Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Article
  2. Article
  3. Frontiers in microbiology · 2022
    Article
  4. Characterization of Extended-Spectrum Beta-Lactamase-ProducingInternational journal of environmental research and public health · 2021
    Article
  5. Phage S144, A New Polyvalent Phage InfectingInternational journal of molecular sciences · 2020
    Article
  6. Analysis of the Molecular Diversity AmongFrontiers in microbiology · 2020
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. DNA-Sequence Based Typing of theFrontiers in microbiology · 2017
    Article
  14. Article
  15. Article
  16. Article
  17. The speciation and genotyping of Cronobacter isolates from hospitalised patients.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

K G JarvisLab 3412, MOD 1 Facility, Virulence Mechanisms Branch (HFS-025), Division of Virulence Assessment, OARSA, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, 8301 Muirkirk Road, Laurel, MD 20708, USA. karen.jarvis@fda.hhs.gov
C J Grim
A A Franco
G Gopinath
V Sathyamoorthy
L Hu
J A Sadowski
C S Lee
B D Tall

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cronobacter (formerly Enterobacter sakazakii) is a recently defined genus consisting of six species, C. sakazakii, C. malonaticus, C. dublinensis, C. muytjensii, C. turicensis, and Cronobacter genomospecies 1. In this study, MboII restriction fragment length polymorphism (RFLP) patterns of O-antigen gene clusters, located between galF and gnd, were used to identify serotypes in Cronobacter spp. Seven O-antigen RFLP clusters were generated, including three C. sakazakii clusters, previously identified as serotypes O1, O2, and O3. The O-antigen regions of six strains with unique RFLP patterns, including two C. sakazakii strains, two C. malonaticus strains, one C. turicensis strain, and one C. muytjensii strain, revealed three O-antigen gene clusters shared among Cronobacter species. PCR assays were developed, targeting the wzx O-antigen polymerase gene, and used to screen 231 Cronobacter strains to determine the frequency of these newly identified serotypes.

Indexed as

Multigene FamilyBacteriological TechniquesCluster AnalysisDNA, BacterialDNA PrimersEnterobacteriaceaeEnterobacteriaceae InfectionsEnvironmental MicrobiologyFood MicrobiologyMolecular Sequence DataO AntigensPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSequence Analysis, DNADNA, BacterialDNA PrimersO Antigens

Identifiers

PMID21531829
PMCPMC3131661

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.