ArticleThe Journal of biological chemistry2011
Phosvitin plays a critical role in the immunity of zebrafish embryos via acting as a pattern recognition receptor and an antimicrobial effector.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 103 citations in OpenAlex.
- Antimicrobial peptide Pt5-1c exerts potent immunomodulatory and antiviral effects by facilitating cellular delivery of cGAMP.Frontiers in immunology · 2026Article
- Maternal regulation of the vertebrate oocyte-to-embryo transition.PLoS genetics · 2024Article
- Illicit Drugs in Surface Waters: How to Get Fish off the Addictive Hook.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Histological and ultrastructural characterization of the dorso-ventral skin of the juvenile and the adult starry puffer fish (Arothron stellatus, Anonymous 1798).BMC veterinary research · 2023Article
- Origin, form and function of extraembryonic structures in teleost fishes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Proteome and Transcriptome Analysis of Gonads Reveals Intersex in Gigantidas haimaensis.BMC genomics · 2022Article
- Article
- Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions.Scientific reports · 2021Article
- Innate Immune Responses of Skin Mucosa in Common Carp (Animals : an open access journal from MDPI · 2020Article
- Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays.Biochemistry research international · 2020Article
- Innate Immunomodulation in Food Animals: Evidence for Trained Immunity?Frontiers in immunology · 2020Review
- Review
- Proteome and Transcriptome Analysis of Ovary, Intersex Gonads, and Testis Reveals Potential Key Sex Reversal/Differentiation Genes and Mechanism in Scallop Chlamys nobilis.Marine biotechnology (New York, N.Y.) · 2018Article
- Recent Advances in Antibacterial and Antiendotoxic Peptides or Proteins from Marine Resources.Marine drugs · 2018Review
- Epidermal mucus, a major determinant in fish health: a review.Iranian journal of veterinary research · 2018Review
- Zebrafish phosvitin-derived peptide Pt5 inhibits melanogenesis via cAMP pathway.Fish physiology and biochemistry · 2017Article
- Identification of the Zinc Finger Protein ZRANB2 as a Novel Maternal Lipopolysaccharide-binding Protein That Protects Embryos of Zebrafish against Gram-negative Bacterial Infections.The Journal of biological chemistry · 2016Article
- Efficient extravasation of tumor-repopulating cells depends on cell deformability.Scientific reports · 2016Article
- Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures.Clinical interventions in aging · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How fish embryos that develop externally survive microbial attacks is poorly understood. Here, we clearly demonstrated that the embryo extract of zebrafish and its early embryo both displayed antimicrobial activity against microbes, including pathogenic Aeromonas hydrophila, and phosvitin (Pv), a nutritional protein abundant in eggs, was related to this antimicrobial activity. We also showed that recombinant Pv (rPv) acted as a pattern recognition receptor capable of recognizing the microbial signature molecules LPS, lipoteichoic acid, and peptidoglycan, as well as binding the Gram-negative and -positive microbes Escherichia coli, A. hydrophila, and Staphylococcus aureus and functioned as an antimicrobial agent capable of killing the microbes. Furthermore, we revealed that its C-terminal 55 residues (Pt5) with the functional sites Arg(242) and Ala(201)/Ile(203) were indispensable for Pv antimicrobial activity. Importantly, microinjection of rPv or Pt5 into early embryos significantly enhanced their resistance to A. hydrophila challenge, and this enhanced bacterial resistance was markedly reduced by co-injection of anti-Pv antibody plus rPv (or Pt5) but not by injection of anti-actin antibody plus rPv. Moreover, the generated mutants with in vitro antimicrobial activity, when injected into the embryos, could also promote their resistance to A. hydrophila, but those without in vitro antimicrobial activity could not. It is thus proposed that Pv participates in the protection of early embryos against pathogenic attacks via binding and disrupting potential pathogens. This work also opens a new way for the study of the immunological roles of yolk proteins in oviparous animals that rely on yolk proteins for embryonic development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.