ArticleThe EMBO journal1990
In vitro splicing of mutually exclusive exons from the chicken beta-tropomyosin gene: role of the branch point location and very long pyrimidine stretch.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 97 citations in OpenAlex.
- Variations of intronic branchpoint motif: identification and functional implications in splicing and disease.Communications biology · 2023Review
- Large-scale analysis of branchpoint usage across species and cell lines.Genome research · 2017Article
- Branch point identification and sequence requirements for intron splicing in Plasmodium falciparum.Eukaryotic cell · 2011Article
- Genome-wide association between branch point properties and alternative splicing.PLoS computational biology · 2010Article
- Identification of motifs that function in the splicing of non-canonical introns.Genome biology · 2008Article
- The polypyrimidine tract binding protein (PTB) represses splicing of exon 6B from the beta-tropomyosin pre-mRNA by directly interfering with the binding of the U2AF65 subunit.Molecular and cellular biology · 2006Article
- A class of human exons with predicted distant branch points revealed by analysis of AG dinucleotide exclusion zones.Genome biology · 2006Article
- Complex splicing pattern generates great diversity in human NF1 transcripts.BMC genomics · 2002Article
- Stability and secretion of acetylcholinesterase forms in skeletal muscle cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Polypyrimidine tract binding protein functions as a repressor to regulate alternative splicing of alpha-actinin mutally exclusive exons.Molecular and cellular biology · 1999Article
- Donor site competition is involved in the regulation of alternative splicing of the rat beta-tropomyosin pre-mRNA.RNA (New York, N.Y.) · 1999Article
- Alternative mRNA splice variants of the rat ClC-2 chloride channel gene are expressed in lung: genomic sequence and organization of ClC-2.Nucleic acids research · 1997Article
- Sequence elements surrounding the acceptor site suppress alternative splicing of the sarco/endoplasmic reticulum Ca2+-ATPase 2 gene transcript.The Biochemical journal · 1997Article
- Functional analysis of the polypyrimidine tract in pre-mRNA splicing.Nucleic acids research · 1997Article
- A T to G mutation in the polypyrimidine tract of the second intron of the human beta-globin gene reduces in vitro splicing efficiency: evidence for an increased hnRNP C interaction.Nucleic acids research · 1995Article
- Polypyrimidine tract sequences direct selection of alternative branch sites and influence protein binding.Nucleic acids research · 1994Article
- Alternative splicing of beta-tropomyosin pre-mRNA: multiple cis-elements can contribute to the use of the 5'- and 3'-splice sites of the nonmuscle/smooth muscle exon 6.Nucleic acids research · 1994Article
- Alternative splicing of pre-mRNAs encoding the nonstructural proteins of minute virus of mice is facilitated by sequences within the downstream intron.Journal of virology · 1994Article
- A novel bipartite splicing enhancer modulates the differential processing of the human fibronectin EDA exon.Nucleic acids research · 1994Article
- cis-elements involved in alternative splicing in the rat beta-tropomyosin gene: the 3'-splice site of the skeletal muscle exon 7 is the major site of blockage in nonmuscle cells.Nucleic acids research · 1993Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chicken beta-tropomyosin gene contains 11 exons, two of which are spliced into mRNA only in skeletal muscle. One pair of alternative exons, 6A and 6B, is found in the middle of the gene; they are spliced in a mutually exclusive manner. The non-muscle splice 6A-7 is by far the predominant in vitro reaction in a HeLa cell nuclear extract. A minor product is the 6A-6B splice, which is excluded in all tissues. This minor product results from the use of a branch point located 105 nt upstream of the 3' end of the intron separating exons 6A and 6B. The region between the branch point sequence and the final AG contains a stretch of approximately 80 pyrimidines. We have examined the role of the distance of the branchpoint to the 3' splice site and of the sequences between these two elements. Our results suggest that at least two cis-acting elements contribute to the mutual exclusivity of exons 6A and 6B. The intron between exons 6A and 6B is intrinsically poorly 'spliceable' both because the branch point is too far upstream of the 3' end of the intron to give efficient splicing and because of the particular sequence lying between this branch point and the 3' splice site.
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