ArticleCurrent biology : CB2011
Proteasome function is required for biological timing throughout the twenty-four hour cycle.
Article in Current biology : CB, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- Circadian Control of Protein Synthesis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Multiomics integration unveils photoperiodic plasticity in the molecular rhythms of marine phytoplankton.The Plant cell · 2025Article
- Generalized Michaelis-Menten rate law with time-varying molecular concentrations.PLoS computational biology · 2023Article
- Studying the Human Microbiota: Advances in Understanding the Fundamentals, Origin, and Evolution of Biological Timekeeping.International journal of molecular sciences · 2023Review
- A phospho-dawn of protein modification anticipates light onset in the picoeukaryote Ostreococcus tauri.Journal of experimental botany · 2023Article
- The proteasome: A key modulator of nervous system function, brain aging, and neurodegenerative disease.Frontiers in cell and developmental biology · 2023Review
- Deep-coverage spatiotemporal proteome of the picoeukaryote Ostreococcus tauri reveals differential effects of environmental and endogenous 24-hour rhythms.Communications biology · 2021Article
- Article
- CRYPTOCHROMES confer robustness, not rhythmicity, to circadian timekeeping.The EMBO journal · 2021Article
- Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells.Journal of biological rhythms · 2019Article
- Article
- Expanding the bioluminescent reporter toolkit for plant science with NanoLUC.Plant methods · 2019Article
- Non-transcriptional processes in circadian rhythm generation.Current opinion in physiology · 2018Review
- Waveforms of molecular oscillations reveal circadian timekeeping mechanisms.Communications biology · 2018Article
- In-depth Characterization of Firefly Luciferase as a Reporter of Circadian Gene Expression in Mammalian Cells.Journal of biological rhythms · 2016Article
- Article
- Circadian Profiling of the Arabidopsis Proteome Using 2D-DIGE.Frontiers in plant science · 2016Article
- Label-free quantitative analysis of the casein kinase 2-responsive phosphoproteome of the marine minimal model species Ostreococcus tauri.Proteomics · 2015Article
- Biochemistry that times the day.Biochemistry · 2015Article
- Circadian clock and photoperiodic response in Arabidopsis: from seasonal flowering to redox homeostasis.Biochemistry · 2015Review
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Abstract
Circadian clocks were, until recently, seen as a consequence of rhythmic transcription of clock components, directed by transcriptional/translational feedback loops (TTFLs). Oscillations of protein modification were then discovered in cyanobacteria. Canonical posttranslational signaling processes have known importance for clocks across taxa. More recently, evidence from the unicellular eukaryote Ostreococcus tauri revealed a transcription-independent, rhythmic protein modification shared in anucleate human cells. In this study, the Ostreococcus system reveals a central role for targeted protein degradation in the mechanism of circadian timing. The Ostreococcus clockwork contains a TTFL involving the morning-expressed CCA1 and evening-expressed TOC1 proteins. Cellular CCA1 and TOC1 protein content and degradation rates are analyzed qualitatively and quantitatively using luciferase reporter fusion proteins. CCA1 protein degradation rates, measured in high time resolution, feature a sharp clock-regulated peak under constant conditions. TOC1 degradation peaks in response to darkness. Targeted protein degradation, unlike transcription and translation, is shown to be essential to sustain TTFL rhythmicity throughout the circadian cycle. Although proteasomal degradation is not necessary for sustained posttranslational oscillations in transcriptionally inactive cells, TTFL and posttranslational oscillators are normally coupled, and proteasome function is crucial to sustain both.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.