ArticleJournal of biomedical optics2011
Comparison of human optimized bacterial luciferase, firefly luciferase, and green fluorescent protein for continuous imaging of cell culture and animal models.
Article in Journal of biomedical optics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- Targeted IL-27-based gene therapy in preventing SARS-CoV-2 entry.Molecular biology reports · 2026Article
- Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression.Nucleic acids research · 2026Article
- Integrating optical coherence tomography and bioluminescence with predictive modeling for quantitative assessment of methicillin-resistantJournal of biomedical optics · 2025Article
- A Gaussia luciferase reporter assay for the evaluation of coronavirus Nsp5/3CLpro activity.Scientific reports · 2024Article
- Comparison of ex vivo bioluminescence imaging, Alu-qPCR and histology for the quantification of spontaneous lung and bone metastases in subcutaneous xenograft mouse models.Clinical & experimental metastasis · 2024Article
- Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer.Scientific reports · 2021Article
- Berberine Reverses Breast Cancer Multidrug Resistance Based on Fluorescence PharmacokineticsACS omega · 2021Article
- Mannosylated Cationic Copolymers for Gene Delivery to Macrophages.Macromolecular bioscience · 2021Article
- Reporter gene comparison demonstrates interference of complex body fluids with secreted luciferase activity.Scientific reports · 2021Article
- Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells.Frontiers in bioengineering and biotechnology · 2021Article
- Article
- Real-time tracking of stem cell viability, proliferation, and differentiation with autonomous bioluminescence imaging.BMC biology · 2020Article
- The antilymphatic metastatic effect of hyaluronic acid in a mouse model of oral squamous cell carcinoma.Cancer biology & therapy · 2020Article
- Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Synthetic metabolic computation in a bioluminescence-sensing system.Nucleic acids research · 2019Article
- Constraints to counting bioluminescence producing cells by a commonly used transgene promoter and its implications for experimental design.Scientific reports · 2019Article
- Strongly enhanced bacterial bioluminescence with theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Discovering Macrophage Functions UsingFrontiers in immunology · 2018Review
- Let there be bioluminescence: development of a biophotonic imaging platform for in situ analyses of oral biofilms in animal models.Environmental microbiology · 2016Article
- The Expanding Toolbox of In Vivo Bioluminescent Imaging.Frontiers in oncology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Bioluminescent and fluorescent reporter systems have enabled the rapid and continued growth of the optical imaging field over the last two decades. Of particular interest has been noninvasive signal detection from mammalian tissues under both cell culture and whole animal settings. Here we report on the advantages and limitations of imaging using a recently introduced bacterial luciferase (lux) reporter system engineered for increased bioluminescent expression in the mammalian cellular environment. Comparison with the bioluminescent firefly luciferase (Luc) system and green fluorescent protein system under cell culture conditions demonstrated a reduced average radiance, but maintained a more constant level of bioluminescent output without the need for substrate addition or exogenous excitation to elicit the production of signal. Comparison with the Luc system following subcutaneous and intraperitoneal injection into nude mice hosts demonstrated the ability to obtain similar detection patterns with in vitro experiments at cell population sizes above 2.5 × 10(4) cells but at the cost of increasing overall image integration time.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.