ArticlePlanta2011
Inhibition of aconitase in citrus fruit callus results in a metabolic shift towards amino acid biosynthesis.
Article in Planta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Molecular orchestration of malate and citrate metabolism: regulatory networks governing organic acid dynamics and fruit quality attributes.Horticulture research · 2026Article
- Transcriptomic Profiling Uncovers Molecular Basis for Sugar and Acid Metabolism in Two Pomegranate (Foods (Basel, Switzerland) · 2025Article
- AP2 transcription factor CsAIL6 negatively regulates citric acid accumulation in citrus fruits by interacting with a WD40 protein CsAN11.Horticulture research · 2025Article
- A full genome assembly reveals drought stress effects on gene expression and metabolite profiles in blackcurrant (Horticulture research · 2025Article
- Comparative Transcriptome Analysis Reveals Potential Molecular Regulation of Organic Acid Metabolism During Fruit Development in Late-Maturing Hybrid Citrus Varieties.International journal of molecular sciences · 2025Article
- ATP Citrate LyasePlants (Basel, Switzerland) · 2024Article
- New Insights into the Genetic Basis of Lysine Accumulation in Rice Revealed by Multi-Model GWAS.International journal of molecular sciences · 2024Article
- Article
- Effects of Nitrogen Deficiency on the Metabolism of Organic Acids and Amino Acids inPlants (Basel, Switzerland) · 2022Article
- Multi-Omics Analysis of Low-Temperature Fruiting Highlights the Promising Cultivation Application of the Nutrients Accumulation inJournal of fungi (Basel, Switzerland) · 2022Article
- Citric Acid-Mediated Abiotic Stress Tolerance in Plants.International journal of molecular sciences · 2021Review
- High-spatiotemporal-resolution transcriptomes provide insights into fruit development and ripening in Citrus sinensis.Plant biotechnology journal · 2021Article
- Citramalate synthase yields a biosynthetic pathway for isoleucine and straight- and branched-chain ester formation in ripening apple fruit.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- ACO2 and ANPEP as novel prognostic markers for gallbladder squamous cell/adenosquamous carcinomas and adenocarcinomas.International journal of clinical oncology · 2020Article
- Review
- Comparative Metabolites and Citrate-Degrading Enzymes Activities in Citrus Fruits Reveal the Role of Balance between ACL and Cyt-ACO in Metabolite Conversions.Plants (Basel, Switzerland) · 2020Article
- Citrus Taste Modification Potentials by Genetic Engineering.International journal of molecular sciences · 2019Review
- Primary Metabolism in Citrus Fruit as Affected by Its Unique Structure.Frontiers in plant science · 2019Review
- Comparative transcriptome and proteome profiling of two Citrus sinensis cultivars during fruit development and ripening.BMC genomics · 2017Article
- Cellular citrate levels establish a regulatory link between energy metabolism and the hepatic iron hormone hepcidin.Journal of molecular medicine (Berlin, Germany) · 2017Article
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Authors and funding
9 authors.
Funding
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Abstract
Citrate, a major determinant of citrus fruit quality, accumulates early in fruit development and declines towards maturation. The isomerization of citrate to isocitrate, catalyzed by aconitase is a key step in acid metabolism. Inhibition of mitochondrial aconitase activity early in fruit development contributes to acid accumulation, whereas increased cytosolic activity of aconitase causes citrate decline. It was previously hypothesized that the block in mitochondrial aconitase activity, inducing acid accumulation, is caused by citramalate. Here, we investigated the effect of citramalate and of another aconitase inhibitor, oxalomalate, on aconitase activity and regulation in callus originated from juice sacs. These compounds significantly increased citrate content and reduced the enzyme's activity, while slightly inducing its protein level. Citramalate inhibited the mitochondrial, but not cytosolic form of the enzyme. Its external application to mandarin fruits resulted in inhibition of aconitase activity, with a transient increase in fruit acidity detected a few weeks later. The endogenous level of citramalate was analyzed in five citrus varieties: its pattern of accumulation challenged the notion of its action as an endogenous inhibitor of mitochondrial aconitase. Metabolite profiling of oxalomalate-treated cells showed significant increases in a few amino acids and organic acids. The activities of alanine transaminase, aspartate transaminase and aspartate kinase, as well as these of two γ-aminobutyrate (GABA)-shunt enzymes, succinic semialdehyde reductase (SSAR) and succinic semialdehyde dehydrogenase (SSAD) were significantly induced in oxalomalate-treated cells. It is suggested that the increase in citrate, caused by aconitase inhibition, induces amino acid synthesis and the GABA shunt, in accordance with the suggested fate of citrate during the acid decline stage in citrus fruit.
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