ArticleMolecular vision2011
Serial analysis of gene expression (SAGE) in normal human trabecular meshwork.
Article in Molecular vision, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 4 of them syntheses that pooled it.
What it found
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Who cites it
16 citing papers in PubMed, 4 syntheses or guidelines pooled it, 28 citations in OpenAlex.
- A multiethnic genome-wide association study of primary open-angle glaucoma identifies novel risk loci.Nature communications · 2018Pooled it
- Genetic association study of exfoliation syndrome identifies a protective rare variant at LOXL1 and five new susceptibility loci.Nature genetics · 2017Pooled it
- Genome-wide association analysis identifies TXNRD2, ATXN2 and FOXC1 as susceptibility loci for primary open-angle glaucoma.Nature genetics · 2016Pooled it
- Meta-analysis of Genome-Wide Association Studies Identifies Novel Loci Associated With Optic Disc Morphology.Genetic epidemiology · 2015Pooled it
- Distribution ofMolecular vision · 2023Article
- A multiethnic genome-wide analysis of 44,039 individuals identifies 41 new loci associated with central corneal thickness.Communications biology · 2020Article
- A large multi-ethnic genome-wide association study identifies novel genetic loci for intraocular pressure.Nature communications · 2017Article
- Major review: Molecular genetics of primary open-angle glaucoma.Experimental eye research · 2017Review
- Transcriptome analysis for the identification of cellular markers related to trabecular meshwork differentiation.BMC genomics · 2017Article
- Transcriptome Analysis on Monocytes from Patients with Neovascular Age-Related Macular Degeneration.Scientific reports · 2016Article
- Identification of an Alu-repeat-mediated deletion of OPTN upstream region in a patient with a complex ocular phenotype.Molecular genetics & genomic medicine · 2015Article
- MicroRNAs that target RGS5.Iranian journal of basic medical sciences · 2015Article
- Gene expression profile in human trabecular meshwork from patients with primary open-angle glaucoma.Investigative ophthalmology & visual science · 2013Article
- Exon-level expression profiling of ocular tissues.Experimental eye research · 2013Article
- Absence of altered expression of optineurin in primary open angle glaucoma patients.Molecular vision · 2012Article
- Non-housekeeping genes expressed in human trabecular meshwork cell cultures.Molecular vision · 2012Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo identify the genes expressed in normal human trabecular meshwork tissue, a tissue critical to the pathogenesis of glaucoma.
methodsTotal RNA was extracted from human trabecular meshwork (HTM) harvested from 3 different donors. Extracted RNA was used to synthesize individual SAGE (serial analysis of gene expression) libraries using the I-SAGE Long kit from Invitrogen. Libraries were analyzed using SAGE 2000 software to extract the 17 base pair sequence tags. The extracted sequence tags were mapped to the genome using SAGE Genie map.
resultsA total of 298,834 SAGE tags were identified from all HTM libraries (96,842, 88,126, and 113,866 tags, respectively). Collectively, there were 107,325 unique tags. There were 10,329 unique tags with a minimum of 2 counts from a single library. These tags were mapped to known unique Unigene clusters. Approximately 29% of the tags (orphan tags) did not map to a known Unigene cluster. Thirteen percent of the tags mapped to at least 2 Unigene clusters. Sequence tags from many glaucoma-related genes, including myocilin, optineurin, and WD repeat domain 36, were identified.
conclusionsThis is the first time SAGE analysis has been used to characterize the gene expression profile in normal HTM. SAGE analysis provides an unbiased sampling of gene expression of the target tissue. These data will provide new and valuable information to improve understanding of the biology of human aqueous outflow.
Indexed as
Identifiers
21528004PMC3081805W61391684What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.