ArticleNature protocols2011
Analysis of methanotroph community composition using a pmoA-based microbial diagnostic microarray.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed.
- The impact of elevated COiScience · 2024Article
- A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments.The ISME journal · 2016Article
- Linking Microbial Community and Catabolic Gene Structures during the Adaptation of Three Contaminated Soils under Continuous Long-Term Pollutant Stress.Applied and environmental microbiology · 2016Article
- Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker.Frontiers in microbiology · 2015Review
- Article
- Termites facilitate methane oxidation and shape the methanotrophic community.Applied and environmental microbiology · 2013Article
- Strain/species-specific probe design for microbial identification microarrays.Applied and environmental microbiology · 2013Article
- Evidence of microbial regulation of biogeochemical cycles from a study on methane flux and land use change.Applied and environmental microbiology · 2013Article
- Methanotrophic bacteria in oilsands tailings ponds of northern Alberta.The ISME journal · 2013Article
- Application of a novel functional gene microarray to probe the functional ecology of ammonia oxidation in nitrifying activated sludge.PloS one · 2013Article
- One millimetre makes the difference: high-resolution analysis of methane-oxidizing bacteria and their specific activity at the oxic-anoxic interface in a flooded paddy soil.The ISME journal · 2012Article
- High-throughput analysis of ammonia oxidiser community composition via a novel, amoA-based functional gene array.PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial diagnostic microarrays (MDMs) are highly parallel hybridization platforms containing multiple sets of immobilized oligonucleotide probes used for parallel detection and identification of many different microorganisms in environmental and clinical samples. Each probe is approximately specific to a given group of organisms. Here we describe the protocol used to develop and validate an MDM method for the semiquantification of a range of functional genes--in this case, particulate methane monooxygenase (pmoA)--and we give an example of its application to the study of the community structure of methanotrophs and functionally related bacteria in the environment. The development and validation of an MDM, following this protocol, takes ∼6 months. The pmoA MDM described in detail comprises 199 probes and addresses ∼50 different species-level clades. An experiment comprising 24 samples can be completed, from DNA extraction to data acquisition, within 3 d (12-13 h bench work).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.