ArticleNature protocols2011
Expressing exogenous genes in newts by transgenesis.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed.
- One-step Cre-loxP organism creation by TAx9.Communications biology · 2025Article
- An adult myogenic cell line of the Japanese fire-bellied newt Cynops pyrrhogaster.Scientific reports · 2024Article
- Development of a ZRS Reporter System for the Newt (Biomedicines · 2024Article
- Newtic1 Is a Component of Globular Structures That Accumulate along the Marginal Band of Erythrocytes in the Limb Blastema of Adult Newt,Biomedicines · 2022Article
- The latent dedifferentiation capacity of newt limb muscles is unleashed by a combination of metamorphosis and body growth.Scientific reports · 2022Article
- The use of transgenics in the laboratory axolotl.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Review
- Skin Wound Healing of the Adult Newt,Biomedicines · 2021Article
- Reviewing the Effects of Skin Manipulations on Adult Newt Limb Regeneration: Implications for the Subcutaneous Origin of Axial Pattern Formation.Biomedicines · 2021Article
- Salamanders: The molecular basis of tissue regeneration and its relevance to human disease.Current topics in developmental biology · 2021Review
- Insights into regeneration tool box: An animal model approach.Developmental biology · 2019Review
- Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt.Scientific reports · 2018Article
- Lens regeneration: a historical perspective.The International journal of developmental biology · 2018Review
- Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts.Scientific reports · 2016Article
- A developmentally regulated switch from stem cells to dedifferentiation for limb muscle regeneration in newts.Nature communications · 2016Article
- The newt (Cynops pyrrhogaster) RPE65 promoter: molecular cloning, characterization and functional analysis.Transgenic research · 2015Article
- The newt reprograms mature RPE cells into a unique multipotent state for retinal regeneration.Scientific reports · 2014Article
- Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination.Nature protocols · 2014Article
- A transcriptome for the study of early processes of retinal regeneration in the adult newt, Cynops pyrrhogaster.PloS one · 2014Article
- Reconstitution of the myocardium in regenerating newt hearts is preceded by transient deposition of extracellular matrix components.Stem cells and development · 2013Article
- Transcriptome analysis of newt lens regeneration reveals distinct gradients in gene expression patterns.PloS one · 2013Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The great regenerative abilities of newts provide the impetus for studies at the molecular level. However, efficient methods for gene regulation have historically been quite limited. Here we describe a protocol for transgenically expressing exogenous genes in the newt Cynops pyrrhogaster. This method is simple: a reaction mixture of I-SceI meganuclease and a plasmid DNA carrying a transgene cassette flanked by the enzyme recognition sites is directly injected into fertilized eggs. The protocol achieves a high efficiency of transgenesis, comparable to protocols used in other animal systems, and it provides a practical number of transgenic newts (∼20% of injected embryos) that survive beyond metamorphosis and that can be applied to regenerative studies. The entire protocol for obtaining transgenic adult newts takes 4-5 months.
Indexed as
Identifiers
21527918What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.