ArticleNature protocols2011
Controlling gene loss of function in newts with emphasis on lens regeneration.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 10 citations in OpenAlex.
- Insights into regeneration tool box: An animal model approach.Developmental biology · 2019Review
- E2F8 as a Novel Therapeutic Target for Lung Cancer.Journal of the National Cancer Institute · 2015Article
- TALEN-mediated gene editing of the thrombospondin-1 locus in axolotl.Regeneration (Oxford, England) · 2015Article
- Lessons learned from vivo-morpholinos: How to avoid vivo-morpholino toxicity.BioTechniques · 2014Article
- A transcriptome for the study of early processes of retinal regeneration in the adult newt, Cynops pyrrhogaster.PloS one · 2014Article
- Transcriptome analysis of newt lens regeneration reveals distinct gradients in gene expression patterns.PloS one · 2013Article
- Inducible genetic system for the axolotl.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Expressing exogenous genes in newts by transgenesis.Nature protocols · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Here we describe a protocol for gene loss of function during regeneration in newts, specifically applied to lens regeneration. Knockdown with the use of morpholinos can be achieved both in vitro and in vivo, depending on the experimental design. These methods achieve desirable levels of gene knockdown, and thus can be compared with methods developed for use in other animals, such as zebrafish. The technology has been applied to study molecular mechanisms during the process of lens regeneration by knocking down genes at specific stages and examining their effects on other genes and lens differentiation. The protocol can take a few days or up to 20 d to complete, depending on the duration of the experiment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.