ArticleGenetics2011
Overcoming redundancy: an RNAi enhancer screen for morphogenesis genes in Caenorhabditis elegans.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis suggests evidence of novel stress-related pathway components in Orsay virus - Caenorhabditis elegans viral model.Scientific reports · 2019Pooled it
- Coincident evolution and functional adaptation of the taxonomically restricted genes ivph-3 and gon-14 in Caenorhabditis nematodes.Biology open · 2025Article
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- Multiplexed CRISPRi Reveals a Transcriptional Switch Between KLF Activators and Repressors in the Maturing Neocortex.bioRxiv : the preprint server for biology · 2025Article
- Tissue-specific RNA-seq defines genes governing male tail tip morphogenesis in C. elegans.Development (Cambridge, England) · 2024Article
- Tissue-specific RNA-seq defines genes governing male tail tip morphogenesis inbioRxiv : the preprint server for biology · 2024Article
- SRGP-1/srGAP and AFD-1/afadin stabilize HMP-1/⍺-catenin at rosettes to seal internalization sites following gastrulation in C. elegans.PLoS genetics · 2023Article
- mRNA localization is linked to translation regulation in theDevelopment (Cambridge, England) · 2020Article
- Review
- Evolutionary Dynamics of the SKN-1 → MED → END-1,3 Regulatory Gene Cascade inG3 (Bethesda, Md.) · 2020Article
- A Transcriptional Lineage of the Early C. elegans Embryo.Developmental cell · 2016Article
- Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure and Caenorhabditis elegans Gastrulation.Genetics · 2016Article
- Asymmetric transcript discovery by RNA-seq in C. elegans blastomeres identifies neg-1, a gene important for anterior morphogenesis.PLoS genetics · 2015Article
- Getting to the core of cadherin complex function in Caenorhabditis elegans.F1000Research · 2015Review
- Challenging muscle homeostasis uncovers novel chaperone interactions in Caenorhabditis elegans.Frontiers in molecular biosciences · 2014Article
- Uncoupling different characteristics of the C. elegans E lineage from differentiation of intestinal markers.PloS one · 2014Article
- Role of sirtuins in lifespan regulation is linked to methylation of nicotinamide.Nature chemical biology · 2013Article
- Cadherins and their partners in the nematode worm Caenorhabditis elegans.Progress in molecular biology and translational science · 2013Review
- A genome-wide RNAi screen for enhancers of par mutants reveals new contributors to early embryonic polarity in Caenorhabditis elegans.Genetics · 2012Article
- Dynamic localization of C. elegans TPR-GoLoco proteins mediates mitotic spindle orientation by extrinsic signaling.Development (Cambridge, England) · 2011Article
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Authors and funding
9 authors.
Funding
Abstract
Morphogenesis is an important component of animal development. Genetic redundancy has been proposed to be common among morphogenesis genes, posing a challenge to the genetic dissection of morphogenesis mechanisms. Genetic redundancy is more generally a challenge in biology, as large proportions of the genes in diverse organisms have no apparent loss of function phenotypes. Here, we present a screen designed to uncover redundant and partially redundant genes that function in an example of morphogenesis, gastrulation in Caenorhabditis elegans. We performed an RNA interference (RNAi) enhancer screen in a gastrulation-sensitized double-mutant background, targeting genes likely to be expressed in gastrulating cells or their neighbors. Secondary screening identified 16 new genes whose functions contribute to normal gastrulation in a nonsensitized background. We observed that for most new genes found, the closest known homologs were multiple other C. elegans genes, suggesting that some may have derived from rounds of recent gene duplication events. We predict that such genes are more likely than single copy genes to comprise redundant or partially redundant gene families. We explored this prediction for one gene that we identified and confirmed that this gene and five close relatives, which encode predicted substrate recognition subunits (SRSs) for a CUL-2 ubiquitin ligase, do indeed function partially redundantly with each other in gastrulation. Our results implicate new genes in C. elegans gastrulation, and they show that an RNAi-based enhancer screen in C. elegans can be used as an efficient means to identify important but redundant or partially redundant developmental genes.
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