ArticleMolecular and cellular biochemistry2011
NADPH regulates human NAD kinase, a NADP⁺-biosynthetic enzyme.
Article in Molecular and cellular biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- NMRK2-YAP-NADK axis preserves redox protection against myocardial ischemia/reperfusion injury.Redox biology · 2026Article
- Collateral lethality of NAD kinase in 1p36 deleted tumors drives vulnerability to NAD kinase 2 depletion.BBA advances · 2026Article
- Oxidative Stress Induces the Phosphorylation of NADNeurochemical research · 2025Article
- NADK tetramer defective mutants affect lung cancer response to chemotherapy via controlling NADK activity.Genes & diseases · 2025Article
- Redox regulation of the immune response.Cellular & molecular immunology · 2022Review
- NADK is activated by oncogenic signaling to sustain pancreatic ductal adenocarcinoma.Cell reports · 2021Article
- Chemo-enzymatic synthesis of adenine substituted nicotinic acid adenine dinucleotide phosphate (NAADP) analogs.Bioorganic & medicinal chemistry · 2021Article
- Mini-Review on Lipofuscin and Aging: Focusing on The Molecular Interface, The Biological Recycling Mechanism, Oxidative Stress, and The Gut-Brain Axis Functionality.Medicina (Kaunas, Lithuania) · 2020Review
- The interplay between oxidative stress and bioenergetic failure in neuropsychiatric illnesses: can we explain it and can we treat it?Molecular biology reports · 2020Review
- Mutant IDH1 Differently Affects Redox State and Metabolism in Glial Cells of Normal and Tumor Origin.Cancers · 2019Article
- Epigenetically dysregulated genes and pathways implicated in the pathogenesis of non-syndromic high myopia.Scientific reports · 2019Article
- Calmodulin Is the Fundamental Regulator of NADK-Mediated NAD Signaling in Plants.Frontiers in plant science · 2019Article
- Emissive Synthetic Cofactors: Enzymatic Interconversions ofAngewandte Chemie (International ed. in English) · 2018Article
- NAD Kinases: Metabolic Targets Controlling Redox Co-enzymes and Reducing Power Partitioning in Plant Stress and Development.Frontiers in plant science · 2018Review
- Nicotinamide is an inhibitor of SIRT1 in vitro, but can be a stimulator in cells.Cellular and molecular life sciences : CMLS · 2017Review
- Identification and characterization of a human mitochondrial NAD kinase.Nature communications · 2012Article
- Structural determinants of discrimination of NAD+ from NADH in yeast mitochondrial NADH kinase Pos5.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NAD kinase (NADK, EC 2.7.1.23) is the sole NADP(+)-biosynthetic enzyme that catalyzes phosphorylation of NAD(+) to yield NADP(+) using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target. Although methods for expression and purification of human NADK have been previously established (Lerner et al. Biochem Biophys Res Commun 288:69-74, 2001), the purification procedure could be significantly improved. In this study, we improved the method for expression and purification of human NADK in Escherichia coli and obtained a purified homogeneous enzyme only through heat treatment and single column chromatography. Using the purified human NADK, we revealed a sigmoidal kinetic behavior toward ATP and the inhibitory effects of NADPH and NADH, but not of NADP(+), on the catalytic activity of the enzyme. These inhibitory effects provide insight into the regulation of intracellular NADPH synthesis. Furthermore, these attributes may provide a clue to design a novel drug against Mycobacterium tuberculosis in which this bacterial NADK is potently inhibited by NADP(+).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.