ArticlePLoS biology2011
A cytoplasmic complex mediates specific mRNA recognition and localization in yeast.
Article in PLoS biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Structural basis for recognition of diverse localizing mRNAs by Egl-BicD.Nature structural & molecular biology · 2026Article
- Intrinsically disordered RNA-binding motifs cooperate to catalyze RNA folding and drive phase separation.Nucleic acids research · 2024Article
- Phosphorylation controls the oligomeric state of She2 and mRNA localization in yeast.RNA (New York, N.Y.) · 2023Article
- The nexus between RNA-binding proteins and their effectors.Nature reviews. Genetics · 2023Review
- A kinesin adapter directly mediates dendritic mRNA localization during neural development in mice.The Journal of biological chemistry · 2020Article
- Expression of Hypoxia-Inducible Factor 1α (HIF-1α) and Genes of Related Pathways in Altered Gravity.International journal of molecular sciences · 2019Article
- Messenger RNA transport in the opportunistic fungal pathogen Candida albicans.Current genetics · 2017Review
- Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region ofThe Journal of biological chemistry · 2017Article
- Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex.Nature structural & molecular biology · 2017Article
- Post-transcriptional gene regulation in the biology and virulence of Candida albicans.Cellular microbiology · 2016Review
- The expanding universe of ribonucleoproteins: of novel RNA-binding proteins and unconventional interactions.Pflugers Archiv : European journal of physiology · 2016Review
- In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.Chemistry & biology · 2015Article
- Hooking She3p onto She2p for myosin-mediated cytoplasmic mRNA transport.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.RNA biology · 2015Article
- A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- In Candida albicans hyphae, Sec2p is physically associated with SEC2 mRNA on secretory vesicles.Molecular microbiology · 2014Article
- Photo-cross-linking and high-resolution mass spectrometry for assignment of RNA-binding sites in RNA-binding proteins.Nature methods · 2014Article
- Co-transcriptional recruitment of Puf6 by She2 couples translational repression to mRNA localization.Nucleic acids research · 2014Article
- Subcellular mRNA localisation at a glance.Journal of cell science · 2014Review
- Dynamic protein ligand interactions--insights from MS.The FEBS journal · 2014Review
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In eukaryotes, hundreds of mRNAs are localized by specialized transport complexes. For localization, transcripts are recognized by RNA-binding proteins and incorporated into motor-containing messenger ribonucleoprotein particles (mRNPs). To date, the molecular assembly of such mRNPs is not well understood and most details on cargo specificity remain unresolved. We used ASH1-mRNA transport in yeast to provide a first assessment of where and how localizing mRNAs are specifically recognized and incorporated into mRNPs. By using in vitro-interaction and reconstitution assays, we found that none of the implicated mRNA-binding proteins showed highly specific cargo binding. Instead, we identified the cytoplasmic myosin adapter She3p as additional RNA-binding protein. We further found that only the complex of the RNA-binding proteins She2p and She3p achieves synergistic cargo binding, with an at least 60-fold higher affinity for localizing mRNAs when compared to control RNA. Mutational studies identified a C-terminal RNA-binding fragment of She3p to be important for synergistic RNA binding with She2p. The observed cargo specificity of the ternary complex is considerably higher than previously reported for localizing mRNAs. It suggests that RNA binding for mRNP localization generally exhibits higher selectivity than inferred from previous in vitro data. This conclusion is fully consistent with a large body of in vivo evidence from different organisms. Since the ternary yeast complex only assembles in the cytoplasm, specific mRNA recognition might be limited to the very last steps of mRNP assembly. Remarkably, the mRNA itself triggers the assembly of mature, motor-containing complexes. Our reconstitution of a major portion of the mRNA-transport complex offers new and unexpected insights into the molecular assembly of specific, localization-competent mRNPs and provides an important step forward in our mechanistic understanding of mRNA localization in general.
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