ArticleJournal of visualized experiments : JoVE2011
Fluorescence recovery after photobleaching (FRAP) of fluorescence tagged proteins in dendritic spines of cultured hippocampal neurons.
Article in Journal of visualized experiments : JoVE, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Excess Met1-linked ubiquitination leads to solid aggregate formation.The EMBO journal · 2026Article
- Increased burden of rare risk variants across gene expression networks predisposes to sporadic Parkinson's disease.Cell reports · 2025Article
- A feedback circuitry involving γ-actin, β-actin and nonmuscle myosin-2 A controls tight junction and apical cortex mechanics.Nature communications · 2025Article
- Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration.Nature communications · 2025Article
- The intrinsically disordered tail of ESCO1 binds DNA in a charge-dependent manner.bioRxiv : the preprint server for biology · 2023Article
- Polo-like kinase-1 mediates hepatitis C virus-induced cell migration, a drug target for liver cancer.Life science alliance · 2023Article
- Actin filaments form a size-dependent diffusion barrier around centrosomes.EMBO reports · 2023Article
- O-glycosylation of the extracellular domain of pollen class I formins modulates their plasma membrane mobility.Journal of experimental botany · 2022Article
- Differences in interaction lead to the formation of different types of insulin amyloid.Scientific reports · 2022Article
- Article
- Dendritic spine loss deep in the neocortex and dendrite distortion with diffusion disturbances occur early in experimental pneumococcal meningitis.Frontiers in neuroscience · 2022Article
- Article
- The Rag GTPase Regulates the Dynamic Behavior of TSC Downstream of Both Amino Acid and Growth Factor Restriction.Developmental cell · 2020Article
- 14-3-3 targets keratin intermediate filaments to mechanically sensitive cell-cell contacts.Molecular biology of the cell · 2020Article
- TLR3 absence confers increased survival with improved macrophage activity against pneumonia.JCI insight · 2019Article
- Direct pulmonary delivery of solubilized curcumin reduces severity of lethal pneumonia.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Activated αScientific reports · 2019Article
- Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.The Journal of biological chemistry · 2019Article
- A centrosomal protein STARD9 promotes microtubule stability and regulates spindle microtubule dynamics.Cell cycle (Georgetown, Tex.) · 2018Article
- Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens.Frontiers in immunology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
FRAP has been used to quantify the mobility of GFP-tagged proteins. Using a strong excitation laser, the fluorescence of a GFP-tagged protein is bleached in the region of interest. The fluorescence of the region recovers when the unbleached GFP-tagged protein from outside of the region diffuses into the region of interest. The mobility of the protein is then analyzed by measuring the fluorescence recovery rate. This technique could be used to characterize protein mobility and turnover rate. In this study, we express the (enhanced green fluorescent protein) EGFP vector in cultured hippocampal neurons. Using the Zeiss 710 confocal microscope, we photobleach the fluorescence signal of the GFP protein in a single spine, and then take time lapse images to record the fluorescence recovery after photobleaching. Finally, we estimate the percentage of mobile and immobile fractions of the GFP in spines, by analyzing the imaging data using ImageJ and Graphpad softwares. This FRAP protocol shows how to perform a basic FRAP experiment as well as how to analyze the data.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.