ArticleJournal of synchrotron radiation2011
Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector.
Article in Journal of synchrotron radiation, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Radiation-induced peroxide rupture and its temperature-dependent repair probed by homogeneous X-ray irradiation.Acta crystallographica. Section D, Structural biology · 2026Article
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- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- Evaluating the impact of X-ray damage on conformational heterogeneity in room-temperature (277 K) and cryo-cooled protein crystals.Acta crystallographica. Section D, Structural biology · 2022Article
- Instrumentation and experimental procedures for robust collection of X-ray diffraction data from protein crystals across physiological temperatures.Journal of applied crystallography · 2020Article
- A shared vision for macromolecular crystallography over the next five years.Structural dynamics (Melville, N.Y.) · 2019Article
- Article
- Revealing the role of microstructure architecture on strength and ductility of Ni microwires by in-situ synchrotron X-ray diffraction.Scientific reports · 2019Article
- VMXi: a fully automated, fully remote, high-flux in situ macromolecular crystallography beamline.Journal of synchrotron radiation · 2019Article
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- Crystal Dehydration in Membrane Protein Crystallography.Advances in experimental medicine and biology · 2016Article
- Challenges and solutions for the analysis of in situ, in crystallo micro-spectrophotometric data.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Temperature-dependent radiation sensitivity and order of 70S ribosome crystals.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Approaches to automated protein crystal harvesting.Acta crystallographica. Section F, Structural biology communications · 2014Article
- A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source.Journal of synchrotron radiation · 2013Article
- A survey of global radiation damage to 15 different protein crystal types at room temperature: a new decay model.Journal of synchrotron radiation · 2013Article
- Sea urchin tooth mineralization: calcite present early in the aboral plumula.Journal of structural biology · 2012Article
- Outrunning free radicals in room-temperature macromolecular crystallography.Acta crystallographica. Section D, Biological crystallography · 2012Article
- Laue crystal structure of Shewanella oneidensis cytochrome c nitrite reductase from a high-yield expression system.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2012Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The first study of room-temperature macromolecular crystallography data acquisition with a silicon pixel detector is presented, where the data are collected in continuous sample rotation mode, with millisecond read-out time and no read-out noise. Several successive datasets were collected sequentially from single test crystals of thaumatin and insulin. The dose rate ranged between ∼ 1320 Gy s(-1) and ∼ 8420 Gy s(-1) with corresponding frame rates between 1.565 Hz and 12.5 Hz. The data were analysed for global radiation damage. A previously unreported negative dose-rate effect is observed in the indicators of global radiation damage, which showed an approximately 75% decrease in D(1/2) at sixfold higher dose rate. The integrated intensity decreases in an exponential manner. Sample heating that could give rise to the enhanced radiation sensitivity at higher dose rate is investigated by collecting data between crystal temperatures of 298 K and 353 K. UV-Vis spectroscopy is used to demonstrate that disulfide radicals and trapped electrons do not accumulate at high dose rates in continuous data collection.
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