ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2011
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 78 citations in OpenAlex.
- Isolation and Characterization of Rabbit Spermatogonial Stem Cells as a Promising Animal Genetic Resource for Biodiversity Protection.Biomolecules · 2026Article
- Single-nucleus RNA sequencing provides insights into the genetic mechanisms underlying reproductive adaptability in Tibetan sheep (Ovis aries).Communications biology · 2026Article
- Spermatogonial Stem Cells in Domestic Animals: Current Insights and Future Directions with a Focus on Dogs.Veterinary sciences · 2025Review
- Identification and Manipulation of Spermatogonial Stem Cells with the Aim of Inducing Spermatogenesis in Vitro.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation†.Biology of reproduction · 2024Review
- Primary Cultures of Spermatogonia and Testis Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A Role for Exchange of Extracellular Vesicles in Porcine Spermatogonial Co-Culture.International journal of molecular sciences · 2022Article
- Article
- Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue.Reproduction & fertility · 2022Article
- Spermatogonial Stem Cell Transplantation in Large Animals.Animals : an open access journal from MDPI · 2021Review
- Metabolic Requirements for Spermatogonial Stem Cell Establishment and Maintenance In Vivo and In Vitro.International journal of molecular sciences · 2021Review
- Recent advances: fertility preservation and fertility restoration options for males and females.Faculty reviews · 2021Review
- Progress inZoological research · 2019Article
- Differential Proliferation Effects after Short-Term Cultivation of Mouse Spermatogonial Stem Cells on Different Feeder Layers.Cell journal · 2019Article
- Generation of functional dopaminergic neurons from human spermatogonial stem cells to rescue parkinsonian phenotypes.Stem cell research & therapy · 2019Article
- Xenotransplantation of canine spermatogonial stem cells (cSSCs) regulated by FSH promotes spermatogenesis in infertile mice.Stem cell research & therapy · 2019Article
- Manipulation of spermatogonial stem cells in livestock species.Journal of animal science and biotechnology · 2019Review
- Biology and manipulation technologies of male germline stem cells in mammals.Reproductive medicine and biology · 2018Review
- Spermatogonial stem cells.Biology of reproduction · 2018Review
- Conserved and non-conserved characteristics of porcine glial cell line-derived neurotrophic factor expressed in the testis.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies suggest that exogenous factors crucial for spermatogonial stem cell (SSC) self-renewal are conserved among several mammalian species. Since glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are critical for rodent SSC self-renewal, we hypothesized that they might promote self-renewal of nonrodent SSCs. Therefore, we cultured testicular germ cells from prepubertal rabbits in the presence of GDNF and FGF2 and found they proliferated indefinitely as cellular clumps that displayed characteristics previously identified for rodent SSCs. The rabbit germ cells could not be maintained on mouse embryonic fibroblast (STO) feeders that support rodent SSC self-renewal in vitro but were rather supported on mouse yolk sac-derived endothelial cell (C166) feeder layers. Proliferation of rabbit germ cells was dependent on GDNF. Of critical importance was that clump-forming rabbit germ cells colonized seminiferous tubules of immunodeficient mice, proliferated for at least 6 mo, while retaining an SSC phenotype in the testes of recipient mice, indicating that they were rabbit SSCs. This study demonstrates that GDNF is a mitogenic factor promoting self-renewal that is conserved between rodent and rabbit SSCs; with an evolutionary separation of ∼ 60 million years. These findings provide a foundation to study the mechanisms governing SSC self-renewal in nonrodent species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.