ArticleAmerican journal of physiology. Cell physiology2011
Rab27b regulates exocytosis of secretory vesicles in acinar epithelial cells from the lacrimal gland.
Article in American journal of physiology. Cell physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 31 citations in OpenAlex.
- Cortical Actin Depolymerisation in 3D Cell Culture Enhances Extracellular Vesicle Secretion and Therapeutic Effects.Journal of extracellular vesicles · 2025Article
- Associations between metabolic disorders and Sjögren's disease.The Japanese dental science review · 2024Review
- Regulation of autophagy by Rab27B in colorectal cancer.The international journal of biochemistry & cell biology · 2024Article
- NSP6 inhibits the production of ACE2-containing exosomes to promote SARS-CoV-2 infectivity.mBio · 2024Article
- Rho and Rab Family Small GTPases in the Regulation of Membrane Polarity in Epithelial Cells.Frontiers in cell and developmental biology · 2022Review
- Morphological and histochemical characterization of the secretory epithelium in the canine lacrimal gland.European journal of histochemistry : EJH · 2021Article
- Rab27a Contributes to Cathepsin S Secretion in Lacrimal Gland Acinar Cells.International journal of molecular sciences · 2021Article
- Biallelic MADD variants cause a phenotypic spectrum ranging from developmental delay to a multisystem disorder.Brain : a journal of neurology · 2020Article
- The GTPase Rab27b regulates the release, autophagic clearance, and toxicity of α-synuclein.The Journal of biological chemistry · 2020Article
- The molecular anatomy of mammalian upper lip and primary palate fusion at single cell resolution.Development (Cambridge, England) · 2019Article
- Molecular Regulatory Mechanism of Exocytosis in the Salivary Glands.International journal of molecular sciences · 2018Review
- The neurotrophin receptor signaling endosome: Where trafficking meets signaling.Developmental neurobiology · 2017Review
- MicroRNA-155 Controls Exosome Synthesis and Promotes Gemcitabine Resistance in Pancreatic Ductal Adenocarcinoma.Scientific reports · 2017Article
- Delivery of Bone Marrow-Derived Mesenchymal Stem Cells Improves Tear Production in a Mouse Model of Sjögren's Syndrome.Stem cells international · 2017Article
- Imbalanced Rab3D versus Rab27 increases cathepsin S secretion from lacrimal acini in a mouse model of Sjögren's Syndrome.American journal of physiology. Cell physiology · 2016Article
- Sequential and compartmentalized action of Rabs, SNAREs, and MAL in the apical delivery of fusiform vesicles in urothelial umbrella cells.Molecular biology of the cell · 2016Article
- Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion.Biochemical and biophysical research communications · 2016Article
- Tear-mediated delivery of nanoparticles through transcytosis of the lacrimal gland.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- A thermo-responsive protein treatment for dry eyes.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Ca²⁺-regulated secretory granule exocytosis in pancreatic and parotid acinar cells.Cell calcium · 2014Review
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
Tear proteins are supplied by the regulated fusion of secretory vesicles at the apical surface of lacrimal gland acinar cells, utilizing trafficking mechanisms largely yet uncharacterized. We investigated the role of Rab27b in the terminal release of these secretory vesicles. Confocal fluorescence microscopy analysis of primary cultured rabbit lacrimal gland acinar cells revealed that Rab27b was enriched on the membrane of large subapical vesicles that were significantly colocalized with Rab3D and Myosin 5C. Stimulation of cultured acinar cells with the secretagogue carbachol resulted in apical fusion of these secretory vesicles with the plasma membrane. Evaluation of morphological changes by transmission electron microscopy of lacrimal glands from Rab27b(-/-) and Rab27(ash/ash)/Rab27b(-/-) mice, but not ashen mice deficient in Rab27a, showed changes in abundance and organization of secretory vesicles, further confirming a role for this protein in secretory vesicle exocytosis. Glands lacking Rab27b also showed increased lysosomes, damaged mitochondria, and autophagosome-like organelles. In vitro, expression of constitutively active Rab27b increased the average size but retained the subapical distribution of Rab27b-enriched secretory vesicles, whereas dominant-negative Rab27b redistributed this protein from membrane to the cytoplasm. Functional studies measuring release of a cotransduced secretory protein, syncollin-GFP, showed that constitutively active Rab27b enhanced, whereas dominant-negative Rab27b suppressed, stimulated release. Disruption of actin filaments inhibited vesicle fusion to the apical membrane but did not disrupt homotypic fusion. These data show that Rab27b participates in aspects of lacrimal gland acinar cell secretory vesicle formation and release.
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