ArticleMolecular biology of the cell2011
Role of AP1 and Gadkin in the traffic of secretory endo-lysosomes.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- Cell signaling and transcriptional regulation of osteoclast lineage commitment, differentiation, bone resorption and diseases.Cell discovery · 2026Review
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- Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor.Nature communications · 2025Article
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- ER-export and ARFRP1/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication.Science advances · 2024Article
- The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells.Journal of virology · 2023Article
- Protein sorting from endosomes to the TGN.Frontiers in cell and developmental biology · 2023Review
- Loss of Christianson Syndrome NaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Article
- Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface.Frontiers in cell and developmental biology · 2020Article
- Cellular and pathophysiological consequences of Arp2/3 complex inhibition: role of inhibitory proteins and pharmacological compounds.Cellular and molecular life sciences : CMLS · 2019Review
- Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells.Journal of lipid research · 2019Article
- Listeriolysin O-dependent host surfaceome remodeling modulates Listeria monocytogenes invasion.Pathogens and disease · 2018Article
- Amyloid precursor protein products concentrate in a subset of exosomes specifically endocytosed by neurons.Cellular and molecular life sciences : CMLS · 2018Article
- Mutant Huntingtin Is Secreted via a Late Endosomal/Lysosomal Unconventional Secretory Pathway.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Alteration of epithelial cell lysosomal integrity induced by bacterial cholesterol-dependent cytolysins.Cellular microbiology · 2017Article
- Protective responses to sublytic complement in the retinal pigment epithelium.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Staccato/Unc-13-4 controls secretory lysosome-mediated lumen fusion during epithelial tube anastomosis.Nature cell biology · 2016Article
- Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation.Scientific reports · 2016Article
- Release of calcium from endolysosomes increases calcium influx through N-type calcium channels: Evidence for acidic store-operated calcium entry in neurons.Cell calcium · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whereas lysosome-related organelles (LRO) of specialized cells display both exocytic and endocytic features, lysosomes in nonspecialized cells can also acquire the property to fuse with the plasma membrane upon an acute rise in cytosolic calcium. Here, we characterize this unconventional secretory pathway in fibroblast-like cells, by monitoring the appearance of Lamp1 on the plasma membrane and the release of lysosomal enzymes into the medium. After sequential ablation of endocytic compartments in living cells, we find that donor membranes primarily derive from a late compartment, but that an early compartment is also involved. Strikingly, this endo-secretory process is not affected by treatments that inhibit endosome dynamics (microtubule depolymerization, cholesterol accumulation, overexpression of Rab7 or its effector Rab-interacting lysosomal protein [RILP], overexpression of Rab5 mutants), but depends on Rab27a, a GTPase involved in LRO secretion, and is controlled by F-actin. Moreover, we find that this unconventional endo-secretory pathway requires the adaptor protein complexes AP1, Gadkin (which recruits AP1 by binding to the γ1 subunit), and AP2, but not AP3. We conclude that a specific fraction of the AP2-derived endocytic pathway is dedicated to secretory purposes under the control of AP1 and Gadkin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.