ArticleJournal of clinical microbiology2011
Use of bexB to detect the capsule locus in Haemophilus influenzae.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 57 citations in OpenAlex.
- Live nontypeable Haemophilus influenzae exacerbates respiratory syncytial virus-associated lower airway inflammation.Respiratory research · 2026Article
- Article
- Molecular and Drug Resistance Characteristics ofMicroorganisms · 2025Article
- Article
- Article
- Clinical epidemiology and impact of Haemophilus influenzae airway infections in adults with cystic fibrosis.BMC infectious diseases · 2024Article
- Article
- Molecular Epidemiology and Antibiotic Resistance Analysis of Non-TypeableAntibiotics (Basel, Switzerland) · 2023Article
- Can non-typeablePediatric investigation · 2023Article
- Epidemic Trends and Biofilm Formation Mechanisms of Haemophilus influenzae: Insights into Clinical Implications and Prevention Strategies.Infection and drug resistance · 2023Review
- The natural history and genetic diversity of Haemophilus influenzae infecting the airways of adults with cystic fibrosis.Scientific reports · 2022Article
- Molecular epidemiology and antimicrobial resistance ofFrontiers in public health · 2022Article
- Nasopharyngeal colonization of otopathogens in South Indian children with acute otitis media - A case control pilot study.Journal of otology · 2021Article
- Epidemiology of Haemophilus influenzae in the Republic of Ireland, 2010-2018.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Widespread of non-typeable Haemophilus influenzae with high genetic diversity after two decades use of Hib vaccine in China.Journal of clinical laboratory analysis · 2020Article
- hicap:Journal of clinical microbiology · 2019Article
- Pneumococcal vaccine impacts on the population genomics of non-typeable Haemophilus influenzae.Microbial genomics · 2018Article
- Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.Emerging infectious diseases · 2018Article
- Encapsulated pleural effusion due toIDCases · 2017Article
- Haemophilus influenzae Type b Invasive Disease in Amish Children, Missouri, USA, 2014.Emerging infectious diseases · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Haemophilus influenzae strains are classified as typeable or nontypeable H. influenzae (NTHI) based upon the presence or absence of capsule. In addition to serotyping, which is subject to false-positive results, typeable strains can be identified through the detection of the capsular export gene bexA and one of six capsule-specific genes, but this method is resource intensive, especially in characterizing large numbers of strains. To address these challenges, we developed a bexB-based method to differentiate true NTHI strains from typeable strains. We validated a PCR-based method to detect bexB in 10 strains whose capsule status was well defined. Among 40 strains that were previously serotype positive in clinical microbiology laboratories, 5 lacked bexA, bexB, and capsule type-specific genes by PCR analysis and thus likely represent false-positive serotyping results. Among 94 additional otitis media, commensal, and serotype b-negative invasive strains, 85 were bexA and bexB negative and 9 contained either a complete or partial capsule locus, i.e., 8 were bexA and bexB positive and 1 was bexA negative but bexB positive. Finally, we adapted the method for use in a high-throughput DNA hybridization-based microarray method, which showed 98.75 and 97.5% concordance to the PCR methods for bexA and bexB, respectively. In addition, bexB showed 84% or greater nucleotide identity among strains containing the capsule locus. In this study, we demonstrate that bexB is a reliable proxy for the capsule locus and that its detection provides a simple and reliable method for differentiating strains that lack the entire capsule locus from those containing a partial or complete capsule locus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.