ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2011
A sensitive alternative for microRNA in situ hybridizations using probes of 2'-O-methyl RNA + LNA.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- Review
- In-situ growth of heterogeneous Au on MoSScientific reports · 2026Article
- Application of microRNA In Situ Hybridization on Long-term Stored Human Formalin-fixed Paraffin-embedded Brain Samples from Psychiatric Patients.Molecular neurobiology · 2025Article
- Identification of a New Role of miR-199a-5p as Factor Implied in Neuronal Damage: Decreasing the Expression of Its Target X-Linked Anti-Apoptotic Protein (XIAP) After SCI.International journal of molecular sciences · 2024Article
- Silencing EGFR-upregulated expression of CD55 and CD59 activates the complement system and sensitizes lung cancer to checkpoint blockade.Nature cancer · 2022Article
- MicroRNA-138-5p Targets Pro-Apoptotic Factors and Favors Neural Cell Survival: Analysis in the Injured Spinal Cord.Biomedicines · 2022Article
- Towards an IdealInternational journal of molecular sciences · 2022Review
- Experimental MicroRNA Detection Methods.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Optimizing locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH) procedure for bacterial detection.PloS one · 2019Article
- High-Quality Genome Assemblies Reveal Long Non-coding RNAs Expressed in Ant Brains.Cell reports · 2018Article
- Anti-miRNA oligonucleotides: A comprehensive guide for design.RNA biology · 2018Review
- Evaluation of fluorescence in situ hybridization techniques to study long non-coding RNA expression in cultured cells.Nucleic acids research · 2018Article
- miR-205 is a critical regulator of lacrimal gland development.Developmental biology · 2017Article
- Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes.Chemical Society reviews · 2017Review
- Inhibition of miR-142-5P ameliorates disease in mouse models of experimental colitis.PloS one · 2017Article
- Article
- A Nuclear Role for miR-9 and Argonaute Proteins in Balancing Quiescent and Activated Neural Stem Cell States.Cell reports · 2016Article
- MiR-130a regulates neurite outgrowth and dendritic spine density by targeting MeCP2.Protein & cell · 2016Article
- Small RNA Detection by in Situ Hybridization Methods.International journal of molecular sciences · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of short, high-affinity probes consisting of a combination of DNA and locked nucleic acid (LNA) has enabled the specific detection of microRNAs (miRNAs) by in situ hybridization (ISH). However, detection of low-copy number miRNAs is still not always possible. Here the authors show that probes consisting of 2'-O-methyl RNAs (2OMe) and LNA at every third base (2:1 ratio), under optimized hybridization conditions, excluding yeast RNA from the hybridization buffer, can provide superior performance in detection of miRNA targets in terms of sensitivity and signal-to-noise ratio compared to DNA + LNA probes. Furthermore, they show that hybridizations can be performed in buffers of 4M urea instead of 50% formamide, thereby yielding an equally specific but nontoxic assay. The use of 2OMe + LNA-based probes and the optimized ISH assay enable simple and fast detection of low-copy number miRNA targets, such as miR-130a in mouse brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.