ArticleAnalytical chemistry2011
Ultrasensitive microarray detection of short RNA sequences with enzymatically modified nanoparticles and surface plasmon resonance imaging measurements.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Plasmonic Film with Diluted Nanostructures for Light Energy Harvesting and Sensing.ACS applied optical materials · 2025Article
- Prospects of Biosensors Based on Functionalized and Nanostructured Solitary Materials: Detection of Viral Infections and Other Risks.ACS omega · 2022Review
- Recent Progresses in Electrochemical DNA Biosensors for MicroRNA Detection.Phenomics (Cham, Switzerland) · 2022Review
- Recent trends in application of nanomaterials for the development of electrochemical microRNA biosensors.Mikrochimica acta · 2021Review
- Recent Advances in Surface Plasmon Resonance Imaging Sensors.Sensors (Basel, Switzerland) · 2019Review
- Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors for In Vitro Diagnostics.International journal of analytical chemistry · 2016Review
- Article
- Surface Enzyme Chemistries for Ultrasensitive Microarray Biosensing with SPR Imaging.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Spherical nucleic acids as a divergent platform for synthesizing RNA-nanoparticle conjugates through enzymatic ligation.ACS nano · 2014Article
- Zeptomole detection of C-reactive protein in serum by a nanoparticle amplified surface plasmon resonance imaging aptasensor.Scientific reports · 2014Article
- Fabrication of DNA microarrays on polydopamine-modified gold thin films for SPR imaging measurements.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- DNAzyme footprinting: detecting protein-aptamer complexation on surfaces by blocking DNAzyme cleavage activity.Journal of the American Chemical Society · 2013Article
- Digital imprinting of RNA recognition and processing on a self-assembled nucleic acid matrix.Scientific reports · 2013Article
- Lessons from human teratomas to guide development of safe stem cell therapies.Nature biotechnology · 2012Review
- Direct quantification of microRNA at low picomolar level in sera of glioma patients using a competitive hybridization followed by amplified voltammetric detection.Analytical chemistry · 2012Article
- Sensitive and label-free biosensing of RNA with predicted secondary structures by a triplex affinity capture method.Nucleic acids research · 2012Article
- Anti-DNA:RNA antibodies and silicon photonic microring resonators: increased sensitivity for multiplexed microRNA detection.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A novel multiplexed method for short RNA detection that employs an enzymatic capture reaction onto DNA-modified silica nanoparticles (SiNPs) followed by nanoparticle-enhanced surface plasmon resonance imaging (SPRI) is demonstrated. SiNPs functionalized with 5'-phosphorylated single stranded DNA (ssDNA) are used with T4 RNA ligase to capture various short 20-24 base single-stranded RNA (ssRNA) oligonucleotides from a target solution. The ssRNA-modified SiNPs are collected from the target solution, specifically adsorbed onto a cDNA microarray and then detected with SPRI. The use of DNA-modified SiNPs to capture ssRNA for profiling has several advantages as compared to a planar SPRI surface bioaffinity adsorption format: (i) the target solution is exposed to a larger total surface area for the RNA ligation reaction; (ii) the SiNPs enhance the diffusion rate of the ssRNA to the surface; (iii) the SiNPs can be collected, washed, and preconcentrated prior to detection; and (iv) the ssRNA-modified SiNPs give an enhanced SPRI signal upon hybridization adsorption to the microarray. Our initial measurements demonstrate that this detection method can be used to detect multiple ssRNA sequences at concentrations as low as 100 fM in 500 μL.
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