ArticleJournal of oral microbiology2010
Differential effects of periopathogens on host protease inhibitors SLPI, elafin, SCCA1, and SCCA2.
Article in Journal of oral microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Synthesis of secretory leukocyte protease inhibitor using cell-free protein synthesis system.Odontology · 2024Article
- Salivary secretory leukocyte protease inhibitor levels in patients with stage 3 grade C periodontitis: a comparative cross-sectional study.Scientific reports · 2022Article
- Neutrophil elastase aggravates periodontitis by disrupting gingival epithelial barrier via cleaving cell adhesion molecules.Scientific reports · 2022Article
- Stable reconstructed human gingiva-microbe interaction model: Differential response to commensals and pathogens.Frontiers in cellular and infection microbiology · 2022Article
- Article
- Multi-species oral biofilm promotes reconstructed human gingiva epithelial barrier function.Scientific reports · 2018Article
- Do oral bacteria alter the regenerative potential of stem cells? A concise review.Journal of cellular and molecular medicine · 2015Review
- Vaginal innate immune mediators are modulated by a water extract of Houttuynia cordata Thunb.BMC complementary and alternative medicine · 2015Article
- Comparative analysis of gingival tissue antigen presentation pathways in ageing and periodontitis.Journal of clinical periodontology · 2014Article
- Are proteinase 3 and cathepsin C enzymes related to pathogenesis of periodontitis?BioMed research international · 2014Article
- Periodontal treatment downregulates protease-activated receptor 2 in human gingival crevicular fluid cells.Infection and immunity · 2013Article
- Changes in bowel microbiota induced by feeding weanlings resistant starch stimulate transcriptomic and physiological responses.Applied and environmental microbiology · 2012Article
- Protease inhibitor levels in periodontal health and disease.Journal of periodontal research · 2012Article
- Periodontal pathogens affect the level of protease inhibitors in gingival crevicular fluid.Molecular oral microbiology · 2012Article
- Filifactor alocis has virulence attributes that can enhance its persistence under oxidative stress conditions and mediate invasion of epithelial cells by porphyromonas gingivalis.Infection and immunity · 2011Article
- The epigenetic paradigm in periodontitis pathogenesis.Journal of Indian Society of PeriodontologyReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveSecretory leukocyte peptidase inhibitors (SLPI), elafin, squamous cell carcinoma antigen 1 and 2 (SCCA1 and SCCA2) are specific endogenous serine protease inhibitors expressed by epithelial cells that prevent tissue damage from excessive proteolytic enzyme activity due to inflammation. To determine the effects of various periopathogens on these protease inhibitors, we utilized human gingival epithelial cells (GECs) challenged with cell-free bacteria supernatants of various periopathogens Porphyromonas gingivalis, Tannerella forsythia, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum.
designThe gene expression and secretion of SLPI, elafin, SCCA1, and SCCA2 were determined using real-time PCR and ELISA, respectively. The direct effects of periopathogens and P. gingivalis gingipain mutants on these inhibitors were examined in vitro by Western Blot. The effect on the innate immune response of GECs was measured by expression of antimicrobial peptides: human beta-defenisin-2 (hBD2) and chemokine (C-C motif) ligand 20 (CCL20).
resultsWe found that SLPI, SCCA2, elafin, hBD2, and CCL20 gene expression levels were significantly induced (p<0.001) in response to P. gingivalis, whose virulence factors include cysteine proteases, but not in response to stimulation by other bacteria. P. gingivalis reduced the secretion of SLPI and elafin significantly in GECs, and degraded recombinant SLPI, elafin, SCCA1, and SCCA2. Differential degradation patterns of SLPI, elafin, SCCA1, and SCCA2 were observed with different bacteria as well as P. gingivalis mutants associated with the loss of specific gingipains secreted by P. gingivalis. In addition, pretreatment of GECs with SLPI, SCCA1, or SCCA2 partially blocked hBD2 and CCL20 mRNA expression in response to P. gingivalis, suggesting a protective effect.
conclusionOur results suggest that different periopathogens affect the host protease inhibitors in a different manner, suggesting host susceptibility may differ in the presence of these pathogens. The balance between cellular protease inhibitors and their degradation may be an important factor in susceptibility to periodontal infection.
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