ArticleBiomicrofluidics2011
Biochip∕laser cell deposition system to assess polarized axonal growth from single neurons and neuron∕glia pairs in microchannels with novel asymmetrical geometries.
Article in Biomicrofluidics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- A Dual-Function Micropatterned Gold Platform for Cell Patterning and Optical Imaging Applications.Sensors and actuators. B, Chemical · 2025Article
- Recent Progress and Perspectives on Neural Chip Platforms Integrating PDMS-Based Microfluidic Devices and Microelectrode Arrays.Micromachines · 2023Review
- Influence of microchannel geometry on device performance and electrophysiological recording fidelity during long-term studies of connected neural populations.Lab on a chip · 2022Article
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- Review
- Nanogroove-Enhanced Hydrogel Scaffolds for 3D Neuronal Cell Culture: An Easy Access Brain-on-Chip Model.Micromachines · 2019Article
- Single-neuron axonal pathfinding under geometric guidance: low-dose-methylmercury developmental neurotoxicity test.Lab on a chip · 2014Article
- Microfluidics-based laser cell-micropatterning system.Biofabrication · 2014Article
- New perspectives on neuronal development via microfluidic environments.Trends in neurosciences · 2012Review
- Cardiogenic Regulation of Stem-Cell Electrical Properties in a Laser-Patterned Biochip.Cellular and molecular bioengineering · 2012Article
- Quantitatively analyzing the protective effect of mesenchymal stem cells on cardiomyocytes in single-cell biochips.Biotechnology letters · 2012Article
- Ultra-rapid laser protein micropatterning: screening for directed polarization of single neurons.Lab on a chip · 2012Article
- A microfluidic platform for controlled biochemical stimulation of twin neuronal networks.Biomicrofluidics · 2012Article
- Laser-patterned stem-cell bridges in a cardiac muscle model for on-chip electrical conductivity analyses.Lab on a chip · 2012Article
- Preface to Special Topic: Biological microfluidics in tissue engineering and regenerative medicine.Biomicrofluidics · 2011Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Axon path-finding plays an important role in normal and pathogenic brain development as well as in neurological regenerative medicine. In both scenarios, axonal growth is influenced by the microenvironment including the soluble molecules and contact-mediated signaling from guiding cells and cellular matrix. Microfluidic devices are a powerful tool for creating a microenvironment at the single cell level. In this paper, an asymmetrical-channel-based biochip, which can be later incorporated into microfluidic devices for neuronal network study, was developed to investigate geometric as well as supporting cell control of polarized axonal growth in forming a defined neuronal circuitry. A laser cell deposition system was used to place single cells, including neuron-glia pairs, into specific microwells of the device, enabling axonal growth without the influence of cytophilic∕phobic surface patterns. Phase microscopy showed that a novel "snag" channel structure influenced axonal growth in the intended direction 4:1 over the opposite direction. In heterotypic experiments, glial cell influence over the axonal growth path was observed with time-lapse microscopy. Thus, it is shown that single cell and heterotypic neuronal path-finding models can be developed in laser patterned biochips.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.