ArticleThe Journal of biological chemistry2011
Relevance of the branch point adenosine, coordination loop, and 3' exon binding site for in vivo excision of the Sinorhizobium meliloti group II intron RmInt1.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Circularization pathway of a bacterial group II intron.Nucleic acids research · 2016Article
- Functionality ofFrontiers in molecular biosciences · 2016Article
- Sequential splicing of a group II twintron in the marine cyanobacterium Trichodesmium.Scientific reports · 2015Article
- Localization of a bacterial group II intron-encoded protein in human cells.Scientific reports · 2015Article
- The Ll.LtrB intron from Lactococcus lactis excises as circles in vivo: insights into the group II intron circularization pathway.RNA (New York, N.Y.) · 2015Article
- In vitro characterization of the splicing efficiency and fidelity of the RmInt1 group II intron as a means of controlling the dispersion of its host mobile element.RNA (New York, N.Y.) · 2014Article
- Insights into the strategies used by related group II introns to adapt successfully for the colonisation of a bacterial genome.RNA biology · 2014Review
- Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.PloS one · 2013Article
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3 authors.
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Abstract
Excision of the bacterial group II intron RmInt1 has been demonstrated in vivo, resulting in the formation of both intron lariat and putative intron RNA circles. We show here that the bulged adenosine in domain VI of RmInt1 is required for splicing via the branching pathway, but branch site mutants produce small numbers of RNA molecules in which the first G residue of the intron is linked to the last C residue. Mutations in the coordination loop in domain I reduced splicing efficiency, but branched templates clearly predominated among splicing products. We also found that a single substitution at the EBS3 position (G329C), preventing EBS3-IBS3 pairing, resulted in the production of 50 to 100 times more RNA molecules in which the 5' and 3' extremities were joined. We provide evidence that these intron molecules may correspond to both, intron circles linked by a 2'-5' phosphodiester bond, and tandem, head-to-tail intron copies.
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