ArticleThe Journal of biological chemistry2011
The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Tail-anchored protein C-terminal domains stimulate ATP hydrolysis by the P5A-ATPase Spf1p.The Journal of biological chemistry · 2026Article
- The azole-resistance phenotype of aMicrobiology spectrum · 2026Article
- Reconstitution of ATP-dependent lipid transporters: gaining insight into molecular characteristics, regulation, and mechanisms.Bioscience reports · 2023Review
- Article
- A Fluorescence-based Approach Utilizing Self-labeling Enzyme Tags to Determine Protein Orientation in Large Unilamellar Vesicles.Bio-protocol · 2022Article
- Single Vesicle Fluorescence-Bleaching Assay for Multi-Parameter Analysis of Proteoliposomes by Total Internal Reflection Fluorescence Microscopy.ACS applied materials & interfaces · 2022Article
- P-Type ATPase Apt1 of the Fungal PathogenJournal of fungi (Basel, Switzerland) · 2021Article
- Functional Significance of Conserved Cysteines in the Extracellular Loops of the ATP Binding Cassette Transporter Pdr11p.Journal of fungi (Basel, Switzerland) · 2020Article
- Membrane Lipid Requirements of the Lysine Transporter Lyp1 from Saccharomyces cerevisiae.Journal of molecular biology · 2020Article
- Steady state analysis of influx and transbilayer distribution of ergosterol in the yeast plasma membrane.Theoretical biology & medical modelling · 2019Article
- The lipid head group is the key element for substrate recognition by the P4 ATPase ALA2: a phosphatidylserine flippase.The Biochemical journal · 2019Article
- Role of lipid transporters in fungal physiology and pathogenicity.Computational and structural biotechnology journal · 2019Review
- Sterol uptake and sterol biosynthesis act coordinately to mediate antifungal resistance in Candida glabrata under azole and hypoxic stress.Molecular medicine reports · 2018Article
- Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniques.European biophysics journal : EBJ · 2017Review
- Purification and characterisation of the yeast plasma membrane ATP binding cassette transporter Pdr11p.PloS one · 2017Article
- ATP-binding cassette transporters and sterol O-acyltransferases interact at membrane microdomains to modulate sterol uptake and esterification.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- A subset of annular lipids is linked to the flippase activity of an ABC transporter.Nature chemistry · 2015Article
- Yeast ABC proteins involved in multidrug resistance.Cellular & molecular biology letters · 2014Review
- Isotope-coded ATP probe for quantitative affinity profiling of ATP-binding proteins.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ATP binding cassette (ABC) transporter Aus1 is expressed under anaerobic growth conditions at the plasma membrane of the yeast Saccharomyces cerevisiae and is required for sterol uptake. These observations suggest that Aus1 promotes the translocation of sterols across membranes, but the precise transport mechanism has yet to be identified. In this study, an extraction and purification procedure was developed to characterize the Aus1 transporter. The detergent-solubilized protein was able to bind and hydrolyze ATP. Mutagenesis of the conserved lysine to methionine in the Walker A motif abolished ATP hydrolysis. Likewise, ATP hydrolysis was inhibited by classical inhibitors of ABC transporters. Upon reconstitution into proteoliposomes, the ATPase activity of Aus1 was specifically stimulated by phosphatidylserine (PS) in a stereoselective manner. We also found that Aus1-dependent sterol uptake, but not Aus1 expression and trafficking to the plasma membrane, was affected by changes in cellular PS levels. These results suggest a direct interaction between Aus1 and PS that is critical for the activity of the transporter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.