ArticleApplied microbiology and biotechnology2011
Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis.
Article in Applied microbiology and biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 188 citations in OpenAlex.
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- CRISPR/Cas9-Mediated Knockout of the Lycopene ε-Cyclase for Efficient Astaxanthin Production in the Green MicroalgaPlants (Basel, Switzerland) · 2024Article
- Microbial chassis as the platform for production of dihydroxy xanthophyll-based carotenoids: an overview of recent advances in biomanufacturing.World journal of microbiology & biotechnology · 2024Review
- Lutein Production and Extraction from Microalgae: Recent Insights and Bioactive Potential.International journal of molecular sciences · 2024Review
- Understanding the Impact of Nitrogen Availability: A Limiting Factor for Enhancing Fucoxanthin Productivity in Microalgae Cultivation.Marine drugs · 2024Article
- Proteomic characterization of a lutein-hyperaccumulating Chlamydomonas reinhardtii mutant reveals photoprotection-related factors as targets for increasing cellular carotenoid content.Biotechnology for biofuels and bioproducts · 2023Article
- Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase.Biotechnology for biofuels and bioproducts · 2023Article
- Roles of Two Phytoene Synthases and Orange Protein in Carotenoid Metabolism of the β-Carotene-AccumulatingMicrobiology spectrum · 2023Article
- Review
- Biochemical Characterization of Orange-Colored Rice Calli Induced by Target Mutagenesis ofPlants (Basel, Switzerland) · 2022Article
- Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production.Current issues in molecular biology · 2022Review
- Physiological and Genetic Regulation for High Lipid Accumulation byMicrobiology spectrum · 2022Article
- Over-expression of a cyanobacterial gene for 1-deoxy-d-xylulose-5-phosphate synthase in the chloroplast ofJournal of King Saud University. Science · 2022Article
- Natural products of medicinal plants: biosynthesis and bioengineering in post-genomic era.Horticulture research · 2022Article
- Origin, evolution, breeding, and omics of Apiaceae: a family of vegetables and medicinal plants.Horticulture research · 2022Article
- Genome-based identification and comparative analysis of enzymes for carotenoid biosynthesis in microalgae.World journal of microbiology & biotechnology · 2021Article
- Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the MicroalgaMarine drugs · 2021Article
- Disparity of the carotenoids antioxidant properties of wild-type andPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The isolation and characterization of the phytoene synthase gene from the green microalga Chlorella zofingiensis (CzPSY), involved in the first step of the carotenoids biosynthetic pathway, have been performed. CzPSY gene encodes a polypeptide of 420 amino acids. A single copy of CzPSY has been found in C. zofingiensis by Southern blot analysis. Heterologous genetic complementation in Escherichia coli showed the ability of the predicted protein to catalyze the condensation of two molecules of geranylgeranyl pyrophosphate (GGPP) to form phytoene. Phylogenetic analysis has shown that the deduced protein forms a cluster with the rest of the phytoene synthases (PSY) of the chlorophycean microalgae studied, being very closely related to PSY of plants. This new isolated gene has been adequately inserted in a vector and expressed in Chlamydomonas reinhardtii. The overexpression of CzPSY in C. reinhardtii, by nuclear transformation, has led to an increase in the corresponding CzPSY transcript level as well as in the content of the carotenoids violaxanthin and lutein which were 2.0- and 2.2-fold higher than in untransformed cells. This is an example of manipulation of the carotenogenic pathway in eukaryotic microalgae, which can open up the possibility of enhancing the productivity of commercial carotenoids by molecular engineering.
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