Evidence map›Paper›PMID 21518957›Full record

ArticleMolecular and cellular biology2011

ck2-dependent phosphorylation of progesterone receptors (PR) on Ser81 regulates PR-B isoform-specific target gene expression in breast cancer cells.

Christy R Hagan, Tarah M Regan, Gwen E Dressing, Carol A Lange

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
5.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 68 citations in OpenAlex.

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  14. Progesterone Receptor Attenuates STAT1-Mediated IFN Signaling in Breast Cancer.Journal of immunology (Baltimore, Md. : 1950) · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Christy R HaganDepartment of Medicine and Women’s Cancer Program, University of Minnesota, Minneapolis, Minnesota, USA.
Tarah M Regan
Gwen E Dressing
Carol A Lange
University of Minnesota · US

Funding

TRAINING GRANT IN MICROBIOLOGY/CANCER RESEARCHT32CA009138 · NCI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI Scott M. Dehm · 1985 to 2026
$11.3M
Progestin and growth factor cross-talk in breast cancerR01CA123763 · NCI · UNIVERSITY OF MINNESOTA · PI LANGE, CAROL A · 2006 to 2010
$1.1M
NCI NIH HHS R01 CA123763NCI NIH HHS T32 CA009138
6 · The paper itself

Abstract

Progesterone receptors (PR) are critical mediators of mammary gland development and contribute to breast cancer progression. Progestin-induced rapid activation of cytoplasmic protein kinases leads to selective regulation of growth-promoting genes by phospho-PR species. Herein, we show that phosphorylation of PR Ser81 is ck2 dependent and progestin regulated in intact cells but also occurs in the absence of PR ligands when cells enter the G(1)/S phase of the cell cycle. T47D breast cancer cells stably expressing a PR-B mutant receptor that cannot be phosphorylated at Ser79/81 (S79/81A) formed fewer soft agar colonies. Regulation of selected genes by PR-B, but not PR-A, also required Ser79/81 phosphorylation for basal and/or progestin-regulated (BIRC3, HSD11β2, and HbEGF) expression. Additionally, wild-type (wt) PR-B, but not S79/81A mutant PR, was robustly recruited to a progesterone response element (PRE)-containing transcriptional enhancer region of BIRC3; abundant ck2 also associated with this region in cells expressing wt but not S79/81A PR. We conclude that phospho-Ser81 PR provides a platform for ck2 recruitment and regulation of selected PR-B target genes. Understanding how ligand-independent PRs function in the context of high levels of kinase activities characteristic of breast cancer is critical to understanding the basis of tumor-specific changes in gene expression and will speed the development of highly selective treatments.

Indexed as

Gene Expression Regulation, Neoplastic11-beta-Hydroxysteroid Dehydrogenase Type 2AnimalsBaculoviral IAP Repeat-Containing 3 ProteinBreast NeoplasmsCasein Kinase IICell Line, TumorFemaleHeparin-binding EGF-like Growth FactorHumansInhibitor of Apoptosis ProteinsIntercellular Signaling Peptides and ProteinsMicePhosphorylationPromoter Regions, GeneticProtein Isoforms11-beta-Hydroxysteroid Dehydrogenase Type 2Baculoviral IAP Repeat-Containing 3 ProteinBIRC3 protein, humanCasein Kinase IIHBEGF protein, humanHbegf protein, mouseHeparin-binding EGF-like Growth FactorHSD11B2 protein, humanInhibitor of Apoptosis ProteinsIntercellular Signaling Peptides and Proteinsprogesterone receptor BProtein IsoformsReceptors, ProgesteroneSerineUbiquitin-Protein Ligases

Identifiers

PMID21518957
PMCPMC3133426
OpenAlexW2139210455

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.