ArticleGut pathogens2011
Real-time PCR analysis of enteric pathogens from fecal samples of irritable bowel syndrome subjects.
Article in Gut pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- Guideline
- Maternal Human Milk Oligosaccharide Profile Modulates the Impact of an Intervention with Iron and Galacto-Oligosaccharides in Kenyan Infants.Nutrients · 2019Trial
- Characterization of toxigenic genes of Bacillus cereus strains isolated from different spices sold in Algeria.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Gut Microbiome-Brain Alliance: A Landscape View into Mental and Gastrointestinal Health and Disorders.ACS chemical neuroscience · 2023Review
- Gut microbiota-stem cell niche crosstalk: A new territory for maintaining intestinal homeostasis.iMeta · 2022Review
- How does Quorum Sensing of Intestinal Bacteria Affect Our Health and Mental Status?Microorganisms · 2022Review
- Article
- Incidence of Cancer in Patients with Irritable Bowl Syndrome.Journal of clinical medicine · 2021Article
- Identification of a Quorum Sensing-Dependent Communication Pathway Mediating Bacteria-Gut-Brain Cross Talk.iScience · 2020Article
- The Role of Intestinal Microbiota and Mast Cell in a Rat Model of Visceral Hypersensitivity.Journal of neurogastroenterology and motility · 2020Article
- Aerobic Mesophilic, Coliform,International journal of environmental research and public health · 2018Article
- Aberrantly Expressed Genes and miRNAs in Slow Transit Constipation Based on RNA-Seq Analysis.BioMed research international · 2018Article
- Close association between intestinal microbiota and irritable bowel syndrome.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
- The Research Progress on Intestinal Stem Cells and Its Relationship with Intestinal Microbiota.Frontiers in immunology · 2017Review
- Wuji Wan Formula Ameliorates Diarrhea and Disordered Colonic Motility in Post-inflammation Irritable Bowel Syndrome Rats by Modulating the Gut Microbiota.Frontiers in microbiology · 2017Article
- Irritable bowel syndrome, the microbiota and the gut-brain axis.Gut microbes · 2016Review
- Rearing Room Affects the Non-dominant Chicken Cecum Microbiota, While Diet Affects the Dominant Microbiota.Frontiers in veterinary science · 2016Article
- Sensitive quantification of Clostridium perfringens in human feces by quantitative real-time PCR targeting alpha-toxin and enterotoxin genes.BMC microbiology · 2015Article
- Recent developments in the pathophysiology of irritable bowel syndrome.World journal of gastroenterology · 2015Review
- Gut microbiota as potential orchestrators of irritable bowel syndrome.Gut and liver · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGrowing amount of scientific evidence suggests that microbes are involved in the pathophysiology of irritable bowel syndrome (IBS). The predominant fecal microbiota composition of IBS subjects has been widely studied with DNA-based techniques but less research has been focused on the intestinal pathogens in this disorder. Here, we optimized a highly sensitive panel of 12 quantitative real-time PCR (qPCR) assays to shed light on the putative presence of intestinal pathogens in IBS sufferers. The panel was used to screen fecal samples from 96 IBS subjects and 23 healthy controls.
resultsFifteen IBS samples (17%) tested positive for Staphylococcus aureus with a thermonuclease (nuc) gene-targeting qPCR assay, whereas none of the healthy controls were positive for S. aureus (p <0.05). The S. aureus -positive IBS samples were confirmed by sequencing of the PCR amplicons. Clostridium perfringens was detected from IBS and control groups with a similar frequency (13% and 17%, respectively) with α-toxin (plc) gene -targeting qPCR assay while none of the samples tested positive for the Cl. perfringens enterotoxin-encoding gene (cpe).
conclusionsThe qPCR panel consisting of 12 assays for an extensive set of pathogenic microorganisms provides an efficient alternative to the conventional detection of gastrointestinal pathogens and could accelerate the initiation of targeted antibiotic therapy reducing the risk of post-infectious IBS (PI-IBS). S. aureus has not been previously reported to be associated with the onset of IBS. Although we discovered significant differences in the prevalence of S. aureus between the study groups, its importance in giving rise to IBS symptoms requires further studies.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.