ArticleTraffic (Copenhagen, Denmark)2011
Dynamics of native β-actin mRNA transport in the cytoplasm.
Article in Traffic (Copenhagen, Denmark), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Amplified MS2 labeling reveals heterogeneous dynamics of RNA granules in the live murine brain.bioRxiv : the preprint server for biology · 2026Article
- Impact of Nuclear Export Pathway on Cytoplasmic HIV-1 RNA Transport Mechanism and Distribution.mBio · 2020Article
- Quantification of native mRNA dynamics in living neurons using fluorescence correlation spectroscopy and reduction-triggered fluorescent probes.The Journal of biological chemistry · 2020Article
- Mechanisms and consequences of subcellular RNA localization across diverse cell types.Traffic (Copenhagen, Denmark) · 2020Review
- Complex Interactions Between Membrane-Bound Organelles, Biomolecular Condensates and the Cytoskeleton.Frontiers in cell and developmental biology · 2020Review
- Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity.eLife · 2019Article
- Crowd Control: Effects of Physical Crowding on Cargo Movement in Healthy and Diseased Neurons.Frontiers in cellular neuroscience · 2019Review
- Engineered mRNA-expressed antibodies prevent respiratory syncytial virus infection.Nature communications · 2018Article
- RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane.Nature communications · 2017Article
- Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA-protein correlations at the level of single cells.Nucleic acids research · 2017Article
- A Novel Method to Quantify RNA-Protein Interactions In Situ Using FMTRIP and Proximity Ligation.Methods in molecular biology (Clifton, N.J.) · 2017Article
- A proximity-dependent assay for specific RNA-protein interactions in intact cells.RNA (New York, N.Y.) · 2016Article
- Imaging viral RNA using multiply labeled tetravalent RNA imaging probes in live cells.Methods (San Diego, Calif.) · 2016Review
- Computing in mammalian cells with nucleic acid strand exchange.Nature nanotechnology · 2016Article
- Can we observe changes in mRNA "state"? Overview of methods to study mRNA interactions with regulatory proteins relevant in cancer related processes.The Analyst · 2016Review
- Cytoplasmic HIV-1 RNA is mainly transported by diffusion in the presence or absence of Gag protein.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Single molecule fluorescence approaches shed light on intracellular RNAs.Chemical reviews · 2014Review
- Article
- Quantifying RNA-protein interactions in situ using modified-MTRIPs and proximity ligation.Nucleic acids research · 2013Article
- Characterization of mRNA-cytoskeleton interactions in situ using FMTRIP and proximity ligation.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Transport of messenger RNAs (mRNAs) in the cytoplasm is essential for localization to translation sites and for post-transcriptional regulation. Utilizing single-RNA sensitive probes and real-time fluorescence microscopy, we accurately quantified the dynamics of native, non-engineered, β-actin mRNAs within the cytoplasm of epithelial cells and fibroblasts for the first time. Using single-particle tracking and temporal analysis, we determined that native β-actin mRNAs, under physiologic conditions, exhibit bursts of intermittent, processive motion on microtubules, interspersed between time periods of diffusive motion, characterized by non-thermal enhanced diffusivity. When transport processes were perturbed via ATP depletion, temperature reduction, dynamitin overexpression and chemical inhibitors, processive motion was diminished or eliminated and diffusivity was reduced. These data support a model whereby processive, motor-driven motion is responsible for long-distance mRNA transport.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.