ArticleACS chemical biology2011
Mechanism of inhibition of Bacillus anthracis spore outgrowth by the lantibiotic nisin.
Article in ACS chemical biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 106 citations in OpenAlex.
- Probiotics and Fermented Foods in Human Nutrition.Molecules (Basel, Switzerland) · 2026Review
- A comparative framework of natural nisin variants: linking genetic basis to future bioengineering applications.Microbial cell factories · 2026Review
- Fast-growingbioRxiv : the preprint server for biology · 2025Article
- From Dormancy to Eradication: Strategies for Controlling Bacterial Persisters in Food Settings.Foods (Basel, Switzerland) · 2025Review
- Strategies for effective high pressure germination or inactivation ofApplied and environmental microbiology · 2024Article
- Synergy Assessment of Four Antimicrobial Bioactive Compounds for the Combinational Treatment of Bacterial Pathogens.Biomedicines · 2023Article
- Nisin E Is a Novel Nisin Variant Produced by MultipleMicroorganisms · 2023Article
- Targeting the Impossible: A Review of New Strategies against Endospores.Antibiotics (Basel, Switzerland) · 2023Review
- Mechanism of Action of Ribosomally Synthesized and Post-Translationally Modified Peptides.Chemical reviews · 2022Review
- Inhibitory effect of licorice extract on the germination and outgrowth ofFrontiers in microbiology · 2022Article
- Inhibitory activities of propolis, nisin, melittin and essential oil compounds onThe journal of venomous animals and toxins including tropical diseases · 2022Article
- Recent Advances in the Application of the Antimicrobial Peptide Nisin in the Inactivation of Spore-Forming Bacteria in Foods.Molecules (Basel, Switzerland) · 2021Review
- Genomic and proteomic comparisons of bacteriocins in probiotic speciesBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Teicoplanin Suppresses VegetativeAntibiotics (Basel, Switzerland) · 2021Article
- Opportunities for Nanomedicine inAntibiotics (Basel, Switzerland) · 2021Review
- Therapeutic Application of Lantibiotics and Other Lanthipeptides: Old and New Findings.Applied and environmental microbiology · 2021Review
- Purification and Characterization of Plantaricin YKX and Assessment of Its Inhibitory Activity AgainstFrontiers in microbiology · 2021Article
- Controlling foodborne pathogens with natural antimicrobials by biological control and antivirulence strategies.Heliyon · 2020Review
- Healthy Human Gastrointestinal Microbiome: Composition and Function After a Decade of Exploration.Digestive diseases and sciences · 2020Review
- Migration of Bacteriocins Across Gastrointestinal Epithelial and Vascular Endothelial Cells, as Determined Using In Vitro Simulations.Scientific reports · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The lantibiotic nisin inhibits growth of vegetative Gram-positive bacteria by binding to lipid II, which disrupts cell wall biosynthesis and facilitates pore formation. Nisin also inhibits the outgrowth of bacterial spores, including spores of Bacillus anthracis, whose structural and biochemical properties are fundamentally different from those of vegetative bacteria. The molecular basis of nisin inhibition of spore outgrowth had not been identified, as previous studies suggested that inhibition of spore outgrowth involved either covalent binding to a spore target or loss of membrane integrity; disruption of cell wall biosynthesis via binding to lipid II had not been investigated. To provide insights into the latter possibility, the effects of nisin were compared with those of vancomycin, another lipid II binding antibiotic that inhibits cell wall biosynthesis but does not form pores. Nisin and vancomycin both inhibited the replication of vegetative cells, but only nisin inhibited the transition from a germinated spore to a vegetative cell. Moreover, vancomycin prevented nisin's activity in competition studies, suggesting that the nisin-lipid II interaction is important for inhibition of spore outgrowth. In experiments with fluorescently labeled nisin, no evidence was found for a covalent mechanism for inhibition of spore outgrowth. Interestingly, mutants in the hinge region (N20P/M21P and M21P/K22P) that still bind lipid II but cannot form pores had potent antimicrobial activity against vegetative B. anthracis cells but did not inhibit spore outgrowth. Therefore, pore formation is essential for the latter activity but not the former. Collectively, these studies suggest that nisin utilizes lipid II as the germinated spore target during outgrowth inhibition and that nisin-mediated membrane disruption is essential to inhibit spore development into vegetative cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.