Evidence map›Paper›PMID 21516491›Full record

ArticleBasic research in cardiology2011

Intracoronary administration of cardiac stem cells in mice: a new, improved technique for cell therapy in murine models.

Qianhong Li, Yiru Guo, Qinghui Ou, Ning Chen, Wen-Jian Wu, Fangping Yuan, Erin O'Brien, Tao Wang, Li Luo, Gregory N Hunt and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Basic research in cardiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed.

  1. Article
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  11. Effects of Heme Oxygenase-1 on c-Kit-Positive Cardiac Cells.International journal of molecular sciences · 2021
    Article
  12. Article
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  16. Review
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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Qianhong LiDivision of Cardiovascular Medicine, University of Louisville, 550 S. Jackson Street, Louisville, KY 40292, USA.
Yiru Guo
Qinghui Ou
Ning Chen
Wen-Jian Wu
Fangping Yuan
Erin O'Brien
Tao Wang
Li Luo
Gregory N Hunt
Xiaoping Zhu
Roberto Bolli

Funding

Tumor Necrosis Factor Modulation of CPCsP01HL078825 · NHLBI · UNIVERSITY OF LOUISVILLE · PI BOLLI, ROBERTO · 2005 to 2021
$37.6M
Mechanisms of CardioprotectionR01HL070897 · NHLBI · UNIVERSITY OF LOUISVILLE · PI BOLLI, ROBERTO · 2003 to 2007
$2.8M
Hematopoietic Stem Cell Therapy for Myocardial RegenerationR01HL076794 · NHLBI · UNIVERSITY OF LOUISVILLE · PI BOLLI, ROBERTO · 2004 to 2007
$1.9M
OXYGEN RADICALS AND CARDIAC ADAPTATIONS TO ISCHEMIAR01HL055757 · NHLBI · UNIVERSITY OF LOUISVILLE · PI BOLLI, ROBERTO · 1995 to 1999
–
NHLBI NIH HHS HL-70897NHLBI NIH HHS HL-76794NHLBI NIH HHS P01 HL078825NHLBI NIH HHS P01HL78825NHLBI NIH HHS R01 HL055757NHLBI NIH HHS R01 HL070897NHLBI NIH HHS R01 HL076794NHLBI NIH HHS R01 HL55757
6 · The paper itself

Abstract

A model of intracoronary stem cell delivery that enables transgenesis/gene targeting would be a powerful tool but is still lacking. To address this gap, we compared intracoronary and intramyocardial delivery of lin(-)/c-kit(+)/GFP(+) cardiac stem cells (CSCs) in a murine model of reperfused myocardial infarction (MI). Lin(-)/c-kit(+)/GFP(+) CSCs were successfully expanded from GFP transgenic hearts and cultured with no detectable phenotypic change for up to ten passages. Intracoronary delivery of CSCs 2 days post-MI resulted in significant alleviation of adverse LV remodeling and dysfunction, which was at least equivalent, if not superior, to that achieved with intramyocardial delivery. Compared with intramyocardial injection, intracoronary infusion was associated with a more homogeneous distribution of CSCs in the infarcted region and a greater increase in viable tissue in this region, suggesting greater formation of new cardiomyocytes. Intracoronary CSC delivery resulted in improved function in the infarcted region, as well as in improved global LV systolic and diastolic function, and in decreased LV dilation and LV expansion index; the magnitude of these effects was similar to that observed after intramyocardial injection. We conclude that, in the murine model of reperfused MI, intracoronary CSC infusion is at least as effective as intramyocardial injection in limiting LV remodeling and improving both regional and global LV function. The intracoronary route appears to be superior in terms of uniformity of cell distribution, myocyte regeneration, and amount of viable tissue in the risk region. To our knowledge, this is the first study to report that intracoronary infusion of stem cells in mice is feasible and effective.

Indexed as

Coronary VesselsModels, AnimalAnimalsCell- and Tissue-Based TherapyCell MovementCells, CulturedFemaleGreen Fluorescent ProteinsInjections, Intra-ArterialMiceMice, Inbred C57BLMice, TransgenicMyocardial InfarctionMyocardiumProto-Oncogene Proteins c-kitRegenerationGreen Fluorescent ProteinsProto-Oncogene Proteins c-kit

Identifiers

PMID21516491
PMCPMC3640450

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.