ArticleMethods in molecular biology (Clifton, N.J.)2011
Real-time cytotoxicity assays.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Protocol for dynamic high-throughput cell death screening of primary phagocytes following microplastic and nanoplastic exposure.STAR protocols · 2025Article
- An Overview of the Current State of Cell Viability Assessment Methods Using OECD Classification.International journal of molecular sciences · 2024Review
- A Fluorescent Real-Time Plaque Assay Enables Single-Cell Analysis of Virus-Induced Cytopathic Effect by Live-Cell Imaging.Viruses · 2021Article
- Drug antagonism and single-agent dominance result from differences in death kinetics.Nature chemical biology · 2020Article
- Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism.Nature communications · 2020Article
- Genome-wide synthetic lethal CRISPR screen identifies FIS1 as a genetic interactor of ALS-linked C9ORF72.Brain research · 2020Article
- Sorting by interfacial tension (SIFT): label-free selection of live cells based on single-cell metabolism.Lab on a chip · 2019Article
- Chromatin Stability as a Target for Cancer Treatment.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Review
- Article
- Real-time cellular exometabolome analysis with a microfluidic-mass spectrometry platform.PloS one · 2015Article
- Limitations of Mild, Moderate, and Profound Hypothermia in Protecting Developing Hippocampal Neurons After Simulated Ischemia.Therapeutic hypothermia and temperature management · 2013Article
- Real-time cell viability assays using a new anthracycline derivative DRAQ7®.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Cytometry of apoptosis. Historical perspective and new advances.Experimental oncology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Validation of new therapeutic targets calls for the advance in innovative assays that probe both spatial and temporal relationships in signaling networks. Cell death assays have already found a widespread use in pharmacological profiling of anticancer drugs. Such assays are, however, predominantly restricted to end point DEAD/LIVE parameter that provides only a snapshot of inherently stochastic process such as tumor cell death. Development of new methods that can offer kinetic real-time analysis would be highly advantageous for the pharmacological screening and predictive toxicology. In the present work we outline innovative protocols for the real-time analysis of tumor cell death, based on propidium iodide (PI) and SYTOX Green probes. These can be readily adapted to both flow cytometry and time-lapse fluorescence imaging. Considering vast time savings and kinetic data acquisition such assays have the potential to be applied in a number of areas including accelerated anticancer drug discovery and high-throughput screening routines.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.