ArticleNature biotechnology2011
Parallel on-chip gene synthesis and application to optimization of protein expression.
Article in Nature biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 153 citations in OpenAlex.
- Engineered eMutS based magnetic separation system for high-fidelity DNA synthesis.Synthetic and systems biotechnology · 2026Article
- High-throughput synthesis of DNA fragments by molecular self-assembly of overlapping oligonucleotides.Nature biotechnology · 2026Article
- Fabrication of microcolumn arrays for high-throughput oligonucleotide synthesis using 3D printing.Scientific reports · 2025Article
- Scaling DNA synthesis with a microchip-based massively parallel synthesis system.Nature biotechnology · 2025Article
- In vitro generation of genetic diversity for directed evolution by error-prone artificial DNA synthesis.Communications biology · 2024Article
- Automated high-throughput DNA synthesis and assembly.Heliyon · 2024Review
- An open-source, 3D printed inkjet DNA synthesizer.Scientific reports · 2024Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Multiplex enzymatic synthesis of DNA with single-base resolution.Science advances · 2023Article
- Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology.Biotechnology journal · 2022Article
- Purification of multiplex oligonucleotide libraries by synthesis and selection.Nature biotechnology · 2022Article
- Glycan chip based on structure-switchable DNA linker for on-chip biosynthesis of cancer-associated complex glycans.Nature communications · 2021Article
- Fluorescent Protein Variants Generated by Reassembly between Skeleton and Chromophore.ACS omega · 2021Article
- Mutations in the Phenicol Exporter GeneFrontiers in microbiology · 2021Article
- Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage.Computational and structural biotechnology journal · 2021Review
- Codon optimization with deep learning to enhance protein expression.Scientific reports · 2020Article
- DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions.Nucleic acids research · 2020Article
- Synthesis of DNA Origami Scaffolds: Current and Emerging Strategies.Molecules (Basel, Switzerland) · 2020Review
- Targeted insertional mutagenesis libraries for deep domain insertion profiling.Nucleic acids research · 2020Article
- Frontiers in Microfluidics, a Teaching Resource Review.Bioengineering (Basel, Switzerland) · 2019Review
14 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Low-cost, high-throughput gene synthesis and precise control of protein expression are of critical importance to synthetic biology and biotechnology. Here we describe the development of an on-chip gene synthesis technology, which integrates on a single microchip the synthesis of DNA oligonucleotides using inkjet printing, isothermal oligonucleotide amplification and parallel gene assembly. Use of a mismatch-specific endonuclease for error correction results in an error rate of ~0.19 errors per kb. We applied this approach to synthesize pools of thousands of codon-usage variants of lacZα and 74 challenging Drosophila protein antigens, which were then screened for expression in Escherichia coli. In one round of synthesis and screening, we obtained DNA sequences that were expressed at a wide range of levels, from zero to almost 60% of the total cell protein mass. This technology may facilitate systematic investigation of the molecular mechanisms of protein translation and the design, construction and evolution of macromolecular machines, metabolic networks and synthetic cells.
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Registered trials
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