ArticleEukaryotic cell2011
Bulk segregant analysis followed by high-throughput sequencing reveals the Neurospora cell cycle gene, ndc-1, to be allelic with the gene for ornithine decarboxylase, spe-1.
Article in Eukaryotic cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 66 citations in OpenAlex.
- The RPD3L deacetylation complex is required for facultative heterochromatin repression inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Use of Bulk Segregant Analysis for Determining the Genetic Basis of Azole Resistance in the Opportunistic PathogenFrontiers in cellular and infection microbiology · 2022Article
- A Major Effect Gene Controlling Development and Pathogenicity inFrontiers in plant science · 2021Article
- Article
- Evolutionarily ancient BAH-PHD protein mediates Polycomb silencing.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Identification of a PRC2 Accessory Subunit Required for Subtelomeric H3K27 Methylation in Neurospora crassa.Molecular and cellular biology · 2020Article
- BSA-Seq Discovery and Functional Analysis of Candidate Hessian Fly (Frontiers in plant science · 2020Article
- Neurospora from Natural Populations: Population Genomics Insights into the Life History of a Model Microbial Eukaryote.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Fifty Aureobasidium pullulans genomes reveal a recombining polyextremotolerant generalist.Environmental microbiology · 2019Article
- Article
- Article
- Article
- Rapid Identification of Major QTLFrontiers in microbiology · 2018Article
- Sources of Fungal Genetic Variation and Associating It with Phenotypic Diversity.Microbiology spectrum · 2017Review
- A Matter of Scale and Dimensions: Chromatin of Chromosome Landmarks in the Fungi.Microbiology spectrum · 2017Review
- EXPLoRA-web: linkage analysis of quantitative trait loci using bulk segregant analysis.Nucleic acids research · 2016Article
- Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei.Microbial cell factories · 2016Review
- Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica.BMC genomics · 2016Article
- Cloning and Characterization ofFrontiers in plant science · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
With the advent of high-throughput DNA sequencing, it is now straightforward and inexpensive to generate high-density small nucleotide polymorphism (SNP) maps. Here we combined high-throughput sequencing with bulk segregant analysis to expedite mutation mapping. The general map location of a mutation can be identified by a single backcross to a strain enriched in SNPs compared to a standard wild-type strain. Bulk segregant analysis simultaneously increases the likelihood of determining the precise nature of the mutation. We present here a high-density SNP map between Neurospora crassa Mauriceville-1-c (FGSC2225) and OR74A (FGSC2489), the strains most typically used by Neurospora researchers to carry out mapping crosses. We further have demonstrated the utility of the Mauriceville sequence and our approach by mapping the mutation responsible for the only existing temperature-sensitive (ts) cell cycle mutation in Neurospora, nuclear division cycle-1 (ndc-1). The single T-to-C point mutation maps to the gene encoding ornithine decarboxylase (ODC), spe-1 (NCU01271), and changes a Phe to a Ser residue within a highly conserved motif next to the catalytic site of the enzyme. By growth on spermidine and complementation with a wild-type spe-1 gene, we showed that the defect in spe-1 is responsible for the ts ndc-1 mutation. Based on our results, we propose changing ndc-1 to spe-1(ndc), which reflects that this mutation results in an ODC with a specific nuclear division defect.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.