ArticleApplied and environmental microbiology2011
Purification, modeling, and analysis of botulinum neurotoxin subtype A5 (BoNT/A5) from Clostridium botulinum strain A661222.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Guideline
- Resolution of Two Steps in Botulinum Neurotoxin Serotype A1 Light Chain Localization to the Intracellular Plasma Membrane.International journal of molecular sciences · 2021Article
- Article
- Review
- Article
- Enhancing toxin-based vaccines against botulism.Vaccine · 2018Article
- Regulation of Botulinum Neurotoxin Synthesis and Toxin Complex Formation by Arginine and Glucose in Clostridium botulinum ATCC 3502.Applied and environmental microbiology · 2017Article
- Entry of Botulinum Neurotoxin Subtypes A1 and A2 into Neurons.Infection and immunity · 2017Article
- Diversity of Group I and II Clostridium botulinum Strains from France Including Recently Identified Subtypes.Genome biology and evolution · 2016Article
- In vivo onset and duration of action varies for botulinum neurotoxin A subtypes 1-5.Toxicon : official journal of the International Society on Toxinology · 2015Article
- Immunoprecipitation of native botulinum neurotoxin complexes from Clostridium botulinum subtype A strains.Applied and environmental microbiology · 2015Article
- Holotoxin Activity of Botulinum Neurotoxin Subtype A4 Originating from a Nontoxigenic Clostridium botulinum Expression System.Applied and environmental microbiology · 2014Article
- Validation of the Endopep-MS method for qualitative detection of active botulinum neurotoxins in human and chicken serum.Analytical and bioanalytical chemistry · 2014Article
- Article
- Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010.PloS one · 2014Article
- Comparison of the catalytic properties of the botulinum neurotoxin subtypes A1 and A5.Biochimica et biophysica acta · 2013Article
- Characterization of botulinum neurotoxin A subtypes 1 through 5 by investigation of activities in mice, in neuronal cell cultures, and in vitro.Infection and immunity · 2013Article
- Purification and characterization of neurotoxin complex from a dual toxin gene containing Clostridium Botulinum Strain PS-5.The protein journal · 2013Article
- Improved detection of botulinum neurotoxin serotype A by Endopep-MS through peptide substrate modification.Analytical biochemistry · 2013Article
- Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
A Clostridium botulinum type A strain (A661222) in our culture collection was found to produce the botulinum neurotoxin subtype A5 (BoNT/A5). Its neurotoxin gene was sequenced to determine its degree of similarity to available sequences of BoNT/A5 and the well-studied BoNT/A1. Thirty-six amino acid differences were observed between BoNT/A5 and BoNT/A1, with the predominant number being located in the heavy chain. The amino acid chain of the BoNT/A from the A661222 strain was superimposed over the crystal structure of the known structure of BoNT/A1 to assess the potential significance of these differences--specifically how they would affect antibody neutralization. The BoNT/A5 neurotoxin was purified to homogeneity and evaluated for certain properties, including specific toxicity and antibody neutralization. This study reports the first purification of BoNTA5 and describes distinct differences in properties between BoNT/A5 and BoNT/A1.
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