ArticleApplied and environmental microbiology2011
Engineering trehalose synthesis in Lactococcus lactis for improved stress tolerance.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Enhancing Probiotic Performance in Food Matrices: From Strain Selection to Bioengineering.Journal of food science · 2026Review
- Trehalose improves cold tolerance ofmSphere · 2026Article
- Inactivation of KhpB (EloR/Jag) inApplied and environmental microbiology · 2025Article
- Development of chemically defined media for3 Biotech · 2023Article
- Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying.Applied microbiology and biotechnology · 2020Article
- Microbial response to acid stress: mechanisms and applications.Applied microbiology and biotechnology · 2020Review
- In a quest for engineering acidophiles for biomining applications: challenges and opportunities.Genes · 2018Review
- Propionibacterium spp.-source of propionic acid, vitamin B12, and other metabolites important for the industry.Applied microbiology and biotechnology · 2018Review
- Probiotic engineering: towards development of robust probiotic strains with enhanced functional properties and for targeted control of enteric pathogens.Gut pathogens · 2017Review
- Cold stress promoting a psychrotolerant bacterium Pseudomonas fragi P121 producing trehaloase.World journal of microbiology & biotechnology · 2016Article
- Interspecies and Intraspecies Analysis of Trehalose Contents and the Biosynthesis Pathway Gene Family Reveals Crucial Roles of Trehalose in Osmotic-Stress Tolerance in Cassava.International journal of molecular sciences · 2016Article
- Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44.Scientific reports · 2016Article
- Effect of Trehalose and Trehalose Transport on the Tolerance ofInternational journal of microbiology · 2016Article
- Dynamic metabolic and transcriptional profiling of Rhodococcus sp. strain YYL during the degradation of tetrahydrofuran.Applied and environmental microbiology · 2014Article
- Trends in bacterial trehalose metabolism and significant nodes of metabolic pathway in the direction of trehalose accumulation.Microbial biotechnology · 2013Review
- Multifaceted attributes of dairy propionibacteria: a review.World journal of microbiology & biotechnology · 2012Review
- Cloning, expression and characterization of trehalose-6-phosphate phosphatase from a psychrotrophic bacterium, Arthrobacter strain A3.World journal of microbiology & biotechnology · 2012Article
- Synthesis, release, and recapture of compatible solute proline by osmotically stressed Bacillus subtilis cells.Applied and environmental microbiology · 2012Article
- Improved trehalose production from biodiesel waste using parent and osmotically sensitive mutant of Propionibacterium freudenreichii subsp. shermanii under aerobic conditions.Journal of industrial microbiology & biotechnology · 2012Article
- A glycine betaine importer limits Salmonella stress resistance and tissue colonization by reducing trehalose production.Molecular microbiology · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trehalose accumulation is a common cell defense strategy against a variety of stressful conditions. In particular, our team detected high levels of trehalose in Propionibacterium freudenreichii in response to acid stress, a result that led to the idea that endowing Lactococcus lactis with the capacity to synthesize trehalose could improve the acid tolerance of this organism. To this end, we took advantage of the endogenous genes involved in the trehalose catabolic pathway of L. lactis, i.e., trePP and pgmB, encoding trehalose 6-phosphate phosphorylase and β-phosphoglucomutase, respectively, which enabled the synthesis of trehalose 6-phosphate. Given that L. lactis lacks trehalose 6-phosphate phosphatase, the respective gene, otsB, from the food-grade organism P. freudenreichii was used to provide the required activity. The trehalose yield was approximately 15% in resting cells and in mid-exponential-phase cells grown without pH control. The intracellular concentration of trehalose reached maximal values of approximately 170 mM, but at least 67% of the trehalose produced was found in the growth medium. The viability of mutant and control strains was examined after exposure to heat, cold or acid shock, and freeze-drying. The trehalose-producing strains showed improved tolerance (5- to 10-fold-higher survivability) to acid (pH 3) and cold shock (4°C); there was also a strong improvement in cell survival in response to heat shock (45°C), and no protection was rendered against dehydration. The insight provided by this work may help the design of food-grade strains optimized for the dairy industry as well as for oral drug delivery.
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