ArticleThe Journal of biological chemistry2011
Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Modulation of SERCA1a by Sesquiterpene Lactones: Insights into Inhibition Mechanisms.Cell biochemistry and biophysics · 2026Article
- Impact of Oxidative Stress and Neuroinflammation on Sarco/Endoplasmic Reticulum CaACS pharmacology & translational science · 2025Article
- Identification of genetic molecular markers and immune infiltration characteristics of Alzheimer's disease through weighted gene co-expression network analysis.Frontiers in neurology · 2022Article
- Shaped by leaky ER: Homeostatic CaFrontiers in physiology · 2022Review
- Structure-function relationships and modifications of cardiac sarcoplasmic reticulum Ca2+-transport.Physiological research · 2021Review
- Maternal high-fat diet associated with altered gene expression, DNA methylation, and obesity risk in mouse offspring.PloS one · 2018Article
- Effects of cyclopiazonic acid and dexamethasone on serotonin-induced calcium responses in vascular smooth muscle cells.Journal of physiology and biochemistry · 2016Article
- Sarcoendoplasmic reticulum Ca(2+) ATPase. A critical target in chlorine inhalation-induced cardiotoxicity.American journal of respiratory cell and molecular biology · 2015Article
- The surging role of Chromogranin A in cardiovascular homeostasis.Frontiers in chemistry · 2014Review
- Regulation of cellular communication by signaling microdomains in the blood vessel wall.Pharmacological reviews · 2014Review
- Microscopic heat pulse-induced calcium dynamics in single WI-38 fibroblasts.Biophysics (Nagoya-shi, Japan) · 2014Article
- Phosphorylated phospholamban stabilizes a compact conformation of the cardiac calcium-ATPase.Biophysical journal · 2013Article
- Interventricular comparison of the energetics of contraction of trabeculae carneae isolated from the rat heart.The Journal of physiology · 2013Article
- Calcium binding and allosteric signaling mechanisms for the sarcoplasmic reticulum Ca²+ ATPase.Protein science : a publication of the Protein Society · 2012Article
- The novel chromogranin A-derived serpinin and pyroglutaminated serpinin peptides are positive cardiac β-adrenergic-like inotropes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Article
- Serpinins: role in granule biogenesis, inhibition of cell death and cardiac function.Current medicinal chemistry · 2012Article
- 2-Color calcium pump reveals closure of the cytoplasmic headpiece with calcium binding.PloS one · 2012Article
- Phospholamban binds with differential affinity to calcium pump conformers.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sarco/endoplasmic reticulum (SR/ER) Ca(2+)-ATPase (SERCA) is an intracellular Ca(2+) pump localized on the SR/ER membrane. The role of SERCA in refilling intracellular Ca(2+) stores is pivotal for maintaining intracellular Ca(2+) homeostasis, and disturbed SERCA activity causes many disease phenotypes, including heart failure, diabetes, cancer, and Alzheimer disease. Although SERCA activity has been described using a simple enzyme activity equation, the dynamics of SERCA activity in living cells is still unknown. To monitor SERCA activity in living cells, we constructed an enhanced CFP (ECFP)- and FlAsH-tagged SERCA2a, designated F-L577, which retains the ATP-dependent Ca(2+) pump activity. The FRET efficiency between ECFP and FlAsH of F-L577 is dependent on the conformational state of the molecule. ER luminal Ca(2+) imaging confirmed that the FRET signal changes directly reflect the Ca(2+) pump activity. Dual imaging of cytosolic Ca(2+) and the FRET signals of F-L577 in intact COS7 cells revealed that SERCA2a activity is coincident with the oscillatory cytosolic Ca(2+) concentration changes evoked by ATP stimulation. The Ca(2+) pump activity of SERCA2a in intact cells can be expressed by the Hill equation with an apparent affinity for Ca(2+) of 0.41 ± 0.0095 μm and a Hill coefficient of 5.7 ± 0.73. These results indicate that in the cellular environment the Ca(2+) dependence of ATPase activation is highly cooperative and that SERCA2a acts as a rapid switch to refill Ca(2+) stores in living cells for shaping the intracellular Ca(2+) dynamics. F-L577 will be useful for future studies on Ca(2+) signaling involving SERCA2a activity.
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