ArticleGenetics2011
Dynamics of a plant RNA virus intracellular accumulation: stamping machine vs. geometric replication.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Stochastic intracellular replication dynamics shape population-level evolution in Influenza A Virus.Virus evolution · 2026Article
- Evolution of viral genomes and their clouds of sequence.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Quasispecies theory and emerging viruses: challenges and applications.Npj viruses · 2024Review
- Decay of HCoV-OC43 infectivity is lower in cell debris-containing media than in fresh culture media.microPublication biology · 2024Article
- Single-cell mutation rate of turnip crinkle virus (-)-strand replication intermediates.PLoS pathogens · 2023Article
- Empirical estimates of the mutation rate for an alphabaculovirus.PLoS genetics · 2022Article
- Article
- A Core35S Promoter of Cauliflower Mosaic Virus Drives More Efficient Replication of Turnip Crinkle Virus.Plants (Basel, Switzerland) · 2021Article
- Why are viral genomes so fragile? The bottleneck hypothesis.PLoS computational biology · 2021Article
- RNA Viral Vectors for Accelerating Plant Synthetic Biology.Frontiers in plant science · 2021Article
- The evolutionary dynamics of viruses: virion release strategies, time delays and fitness minima.Virus evolution · 2021Article
- Impact of genetic drift, selection and accumulation level on virus adaptation to its host plants.Molecular plant pathology · 2018Article
- Interferon at the cellular, individual, and population level in hepatitis C virus infection: Its role in the interferon-free treatment era.Immunological reviews · 2018Review
- Noise-induced bistability in the quasi-neutral coexistence of viral RNAs under different replication modes.Journal of the Royal Society, Interface · 2018Article
- A New Mechanistic Model for Viral Cross Protection and Superinfection Exclusion.Frontiers in plant science · 2018Review
- The virome ofVirus evolution · 2018Article
- Mechanisms of viral mutation.Cellular and molecular life sciences : CMLS · 2016Review
- Predicting the Stability of Homologous Gene Duplications in a Plant RNA Virus.Genome biology and evolution · 2016Article
- Single-Cell Analysis of RNA Virus Infection Identifies Multiple Genetically Diverse Viral Genomes within Single Infectious Units.Cell host & microbe · 2015Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tremendous evolutionary potential of RNA viruses allows them to thrive despite host defense mechanisms and endows them with properties such as emergence, host switching, and virulence. The frequency of mutant viruses after an infectious process results from the interplay between the error rate of the viral replicase, from purifying mechanisms acting after transcription on aberrant RNAs, and from the amplification dynamics of virus RNA positive (+) and negative (-) strands. Two extreme scenarios describing viral RNA amplification are the geometric growth, in which each RNA strand serves as template for the synthesis of complementary strands with the same efficiency, and the stamping machine, where a strand is reiteratively used as template to synthesize multiple copies of the complementary. The resulting mutation frequencies are completely different, being geometric growth largely more mutagenic than stamping machine. In this work we evaluate the contribution of geometric growth and stamping machine to the overall genome amplification of the plant (+)-strand RNA virus turnip mosaic virus (TuMV). By means of transfection experiments of Nicotiana benthamiana protoplasts with a TuMV cDNA infectious clone and by using strand-specific quantitative real-time PCR, we determined the amplification dynamics of viral (+) and (-) RNA during a single-cell infectious process. A mathematical model describing the amplification of each viral strand was fitted to the data. Analyses of the model parameters showed that TuMV (+) and (-) RNA amplification occurs through a mixed strategy with ∼93% of genomes produced via stamping machine and only ∼7% resulting from geometric growth.
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