Evidence map›Paper›PMID 21515433›Full record

ArticleJournal of proteomics2011

Determination of the Plasmodium vivax schizont stage proteome.

Wanlapa Roobsoong, Sittiruk Roytrakul, Jetsumon Sattabongkot, Jianyong Li, Rachanee Udomsangpetch, Liwang Cui

Abstract read
In one paragraph

Article in Journal of proteomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
4.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 39 citations in OpenAlex.

  1. Article
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  4. The Black Box of Cellular and Molecular Events ofInternational journal of molecular sciences · 2022
    Review
  5. Article
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  7. Article
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  9. Frontiers in cellular and infection microbiology · 2018
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 5 institutions in 2 countries.

Wanlapa RoobsoongFaculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.
Sittiruk Roytrakul
Jetsumon Sattabongkot
Jianyong Li
Rachanee Udomsangpetch
Liwang Cui
Mahidol University · THArmed Forces Research Institute of Medical Science · THNational Center for Genetic Engineering and Biotechnology · THPennsylvania State University · USVirginia Tech · US

Funding

Vivax Malaria culture technique and genetic diversity in ThailandD43TW006571 · FIC · PENNSYLVANIA STATE UNIVERSITY-UNIV PARK · PI CUI, LIWANG · 2004 to 2018
$1.9M
Plasmodium vivax:Proteomic Analysis of Asexual and Sporozoite StagesR21AI069126 · NIAID · PENNSYLVANIA STATE UNIVERSITY, THE · PI CUI, LIWANG · 2006 to 2007
$393k
FIC NIH HHS D43 TW006571FIC NIH HHS D43-TW006571NIAID NIH HHS R21 AI069126NIAID NIH HHS R21AI069126
6 · The paper itself

Abstract

With the genome of the malaria parasite Plasmodium vivax sequenced, it is important to determine the proteomes of the parasite in order to assist efforts in antigen and drug target discovery. Since a method for continuous culture of P. vivax parasite is not available, we tried to study the proteome of the erythrocytic stages using fresh parasite isolates from patients. In schizont-enriched samples, 316 proteins were confidently identified by tandem mass spectrometry. Almost 50% of the identified proteins were hypothetical, while other major categories include proteins with binding function, protein fate, protein synthesis, metabolism and cellular transport. To identify proteins that are recognized by host humoral immunity, parasite proteins were separated by two-dimensional gel electrophoresis and screened by Western blot using an immune serum from a P. vivax patient. Mass spectrometry analysis of protein spots recognized by the serum identified four potential antigens including PV24. The recombinant protein PV24 was recognized by antibodies from vivax malaria patients even during the convalescent period, indicating that PV24 could elicit long-lasting antibody responses in P. vivax patients.

Indexed as

Antibodies, ProtozoanAntigens, ProtozoanErythrocytesHumansImmunity, HumoralPlasmodium vivaxProteomeProtozoan ProteinsSerologic TestsTandem Mass SpectrometryAntibodies, ProtozoanAntigens, ProtozoanProteomeProtozoan Proteins

Identifiers

PMID21515433
PMCPMC3156846
OpenAlexW1993401874

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.