ArticleBiomaterials2011
Gene therapy vectors with enhanced transfection based on hydrogels modified with affinity peptides.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Chitosan Hydrogel-Delivered ABE8e Corrects PAX9 Mutant in Dental Pulp Stem Cells.Gels (Basel, Switzerland) · 2023Article
- Hydrogel-Based Localized Nonviral Gene Delivery in Regenerative Medicine Approaches-An Overview.Pharmaceutics · 2020Review
- Enhanced wound healing via collagen-turnover-driven transfer of PDGF-BB gene in a murine wound model.ACS applied bio materials · 2020Article
- Hydrogel-Based Controlled Delivery Systems for Articular Cartilage Repair.BioMed research international · 2016Article
- Opportunities for multicomponent hybrid hydrogels in biomedical applications.Biomacromolecules · 2015Review
- Heparin-chitosan nanoparticle functionalization of porous poly(ethylene glycol) hydrogels for localized lentivirus delivery of angiogenic factors.Biomaterials · 2014Article
- Engineering peripheral nerve repair.Current opinion in biotechnology · 2013Review
- Matrix-based gene delivery for tissue repair.Current opinion in biotechnology · 2013Review
- Biomaterial Scaffolds for Controlled, Localized Gene Delivery of Regenerative Factors.Advances in wound care · 2013Review
- Hydrogels for lentiviral gene delivery.Expert opinion on drug delivery · 2013Review
- Hydrogel macroporosity and the prolongation of transgene expression and the enhancement of angiogenesis.Biomaterials · 2012Article
- Hydrogel design for supporting neurite outgrowth and promoting gene delivery to maximize neurite extension.Biotechnology and bioengineering · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Regenerative strategies for damaged tissue aim to present biochemical cues that recruit and direct progenitor cell migration and differentiation. Hydrogels capable of localized gene delivery are being developed to provide a support for tissue growth, and as a versatile method to induce the expression of inductive proteins; however, the duration, level, and localization of expression is often insufficient for regeneration. We thus investigated the modification of hydrogels with affinity peptides to enhance vector retention and increase transfection within the matrix. PEG hydrogels were modified with lysine-based repeats (K4, K8), which retained approximately 25% more vector than control peptides. Transfection increased 5- to 15-fold with K8 and K4 respectively, over the RDG control peptide. K8- and K4-modified hydrogels bound similar quantities of vector, yet the vector dissociation rate was reduced for K8, suggesting excessive binding that limited transfection. These hydrogels were subsequently applied to an in vitro co-culture model to induce NGF expression and promote neurite outgrowth. K4-modified hydrogels promoted maximal neurite outgrowth, likely due to retention of both the vector and the NGF. Thus, hydrogels modified with affinity peptides enhanced vector retention and increased gene delivery, and these hydrogels may provide a versatile scaffold for numerous regenerative medicine applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.