ArticleThe American journal of pathology2011
Adipose tissue-derived stem cell in vitro differentiation in a three-dimensional dental bud structure.
Article in The American journal of pathology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 40 citations in OpenAlex.
- Pooled it
- Cartilage Regeneration in Human with Adipose Tissue-Derived Stem Cells: Current Status in Clinical Implications.BioMed research international · 2016Pooled it
- Micro-fragmented adipose tissue-An innovative therapeutic approach: A narrative review.Medicine · 2025Review
- Tissue Regeneration on Rise: Dental Hard Tissue Regeneration and Challenges-A Narrative Review.Scientifica · 2024Review
- Considering the Value of 3D Cultures for Enhancing the Understanding of Adhesion, Proliferation, and Osteogenesis on Titanium Dental Implants.Biomolecules · 2023Article
- Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells.Journal of functional biomaterials · 2022Article
- Role and application of stem cells in dental regeneration: A comprehensive overview.EXCLI journal · 2021Review
- Tooth Formation: Are the Hardest Tissues of Human Body Hard to Regenerate?International journal of molecular sciences · 2020Review
- Differentiation of Human Mesenchymal Stem Cells towards Neuronal Lineage: Clinical Trials in Nervous System Disorders.Biomolecules & therapeutics · 2020Review
- Adipose Derived Stem Cells for Corneal Wound Healing after Laser Induced Corneal Lesions in Mice.Journal of clinical medicine · 2017Article
- Current use of autologous adipose tissue-derived stromal vascular fraction cells for orthopedic applications.Journal of biomedical science · 2017Review
- Potential use of mesenchymal stem cells in human meniscal repair: current insights.Open access journal of sports medicine · 2017Review
- Odontogenic epithelial stem cells: hidden sources.Laboratory investigation; a journal of technical methods and pathology · 2015Review
- Ultrastructural analysis of different human mesenchymal stem cells after in vitro expansion: a technical review.European journal of histochemistry : EJH · 2015Article
- A comparison of the in vitro mineralisation and dentinogenic potential of mesenchymal stem cells derived from adipose tissue, bone marrow and dental pulp.Journal of bone and mineral metabolism · 2015Article
- Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art.Cell regeneration (London, England) · 2015Article
- Regenerative repair of damaged meniscus with autologous adipose tissue-derived stem cells.BioMed research international · 2014Review
- Adipose tissue stem cells: the great WAT hope.Trends in endocrinology and metabolism: TEM · 2012Review
- Adipose tissue stem cells meet preadipocyte commitment: going back to the future.Journal of lipid research · 2012Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tooth morphogenesis requires sequential and reciprocal interactions between the cranial neural crest-derived mesenchymal cells and the stomadial epithelium, which regulate tooth morphogenesis and differentiation. We show how mesenchyme-derived single stem cell populations can be induced to transdifferentiate in vitro in a structure similar to a dental bud. The presence of stem cells in the adipose tissue has been previously reported. We incubated primary cultures of human adipose tissue-derived stem cells in a dental-inducing medium and cultured the aggregates in three-dimensional conditions. Four weeks later, cells formed a three-dimensional organized structure similar to a dental bud. Expression of dental tissue-related markers was tested assaying lineage-specific mRNA and proteins by RT-PCR, immunoblot, IHC, and physical-chemical analysis. In the induction medium, cells were positive for ameloblastic and odontoblastic markers as both mRNAs and proteins. Also, cells expressed epithelial, mesenchymal, and basement membrane markers with a positional relationship similar to the physiologic dental morphogenesis. Physical-chemical analysis revealed 200-nm and 50-nm oriented hydroxyapatite crystals as displayed in vivo by enamel and dentin, respectively. In conclusion, we show that adipose tissue-derived stem cells in vitro can transdifferentiate to produce a specific three-dimensional organization and phenotype resembling a dental bud even in the absence of structural matrix or scaffold to guide the developmental process.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.